Professor David Tscharke
Areas of expertise
- Virology 060506
- Medical Virology 110804
- Immunology 1107
- Cellular Immunology 110704
- Gene And Molecular Therapy 100401
- Immunogenetics (Incl. Genetic Immunology) 110706
- Medical Molecular Engineering Of Nucleic Acids And Proteins 100403
- Infectious Diseases 110309
Research interests
The overall theme of my research is to understand the interactions between viruses and our body. This includes how viruses cause disease and how our immune system combats viruses. It also includes the biology of vaccines.
- Herpes simplex virus (and herpesviruses in general), especially latency
- Antigen presentation, especially to CD8+ T cells
- Vaccinia virus as a vaccine vector
- Vaccinia virus (and poxviruses in general)
Biography
David Tscharke began his research career at the University of Adelaide and IMVS in Adelaide, studying interactions between the immune system and herpes simplex virus (HSV, the cause of cold sores). After gaining his PhD he worked in postdoctoral positions at Oxford University and then Imperial College London in the UK, working on projects related to viral pathogenesis and vaccine design. Following this, he moved to the US National Institutes of Health (Bethesda, MD) where he focused on understanding how the immune system recognises viruses and vaccines. He then returned to Australia, working first at QIMR in Brisbane, before starting his own lab and taking on undergraduate teaching in the former department of Biochemistry and Molecular Biology at the ANU in 2006. Since then he has received a Young Tall Poppy Award and has held an NHMRC Career Development Award and ARC Future Fellowship. In 2016 he moved his laboratory across campus to the John Curtin School of Medical Research (JCSMR) and took up an NHMRC Senior Research Fellowship. Since mid-2017 he has been Head of the Department of Immunology and Infectious Diseases at JCSMR. Throughout his career he has had the benefit of working with excellent mentors, generous collaborators and brilliant students. His current research combines all the themes he has developed throughout his career, from understanding the dormant phase of infection with HSV to gaining new insight into the way our immune system recognises and responds to viruses and vaccines.
Researcher's projects
Understanding the role of ongoing viral activity in herpes simplex virus latency
Herpes simplex viruses (HSV) type 1 and 2 are highly prevalent pathogens that cause substantial morbidity and occasional deaths. Recurrent ocular infections can lead to blindness and increasing genital HSV-1 infections and shedding during childbirth are a growing risk to neonates. These two important clinical situations highlight the importance of understanding HSV latency and the significance of any therapy that could inhibit the reactivation of the virus. Despite this importance, the mechanisms controlling the HSV latency remain poorly understood. In part this is due to an understanding of latent infection that emphasises the importance of the restriction of viral gene expression. Our finding that multiple HSV genes can be expressed during latency and that neurons are responding, suggests a new paradigm and hope for therapeutic interventions that boost the ability of the host to maintain latent infection. The way forward is to 1) further explore this new paradigm in our highly tractable mouse model, 2) to translate it to human neurons and then 3) identify and test host pathways that restrict HSV infection. This approach forms the aims of this proposal. Ultimately the pathways we identify might yield targets for future therapies that aim to keep HSV latency truly silent.
Recognition of virus-infected cells by CD8+ T cells
Hypothesis: A systematic approach to immunopeptidomes and their immunogenicity will provide insights for anti-viral immunity, vaccine design and mechanisms of autoimmunity.
Viral infections constitute a significant health burden world-wide and a better understanding of anti-viral immunity is required to improve vaccines. The other side of effective immunity is autoimmunity, and there is a long history of association between infection and autoimmune diseases. In each case a clearer understanding of the interaction between pathogens and the immune system is needed. One of the most vital interfaces between host and virus is the display of virus peptides in the context of MHC I on infected cells and the response of CD8+ T cells. Despite this clear significance, our understanding of the full range of peptides that are presented (or immunopeptidomes) and how they shape the specificity
of anti-viral CD8+ T cell responses remains highly fragmented. We are exploring these issues using vaccinia virus, arguably the most successful human vaccine and also a virus for which we have well characterised models in mice, enabling the quantitative study of immunopeptidomes and responding T cells. Using this model we are establishing and comparing detailed immunopeptidomes for vaccinia virus on cell lines, primary cells and cells from infected mice. Next we are determining what fraction of an immunopeptidome elicits CD8+ T cell responses. We are also investigating whether central tolerance mechanisms narrow the range of epitopes targeted by anti-viral immunity and whether immunodomination and competition amongst peptides for presentation reduces immunogenicity of some epitopes.
Available student projects
Equiries are welcome from potential honours or PhD students with an inteterst in topics at the interface between virology and immunology, and especially antigen presentation to CD8+ T cells. A variety of projects are available, from the molecular virology of HSV latency to quantitative studies of antigen presentation on class I MHC. Prospective students are encouraged to read our recent publications and contact David if there is a good fit between your interest and our areas of research.
Publications
- Ashok, D, Taheri, M, Garg, P et al. 2022, 'Shielding Surfaces from Viruses and Bacteria with a Multiscale Coating', Advanced Science, vol. online.
- Purohit, S, Samer, C, McWilliam, H et al. 2021, 'Varicella Zoster Virus Impairs Expression of the Nonclassical Major Histocompatibility Complex Class I– Related Gene Protein (MR1)', The Journal of Infectious Diseases, vol. 2021, pp. 1-11.
- Gowripalan, A, Smith, S & Tscharke, D 2021, 'Selection of Vaccinia Virus Recombinants Using CRISPR/Cas9', Bio-Protocol, vol. 11, no. 24, pp. 1-15.
- Tseng, Y, Croft, S, Smith, S et al. 2021, 'Viperin has species-specific roles in response to herpes simplex virus infection', Journal of General Virology, vol. 102, no. 8.
- Jurgens, A, Tscharke, D & Brocks, J 2021, 'FROM CALIGARI TO JOKER: the clown prince of crime's psychopathic science', Journal of Graphic Novels and Comics, vol. Online, pp. 1-16.
- Jurgens, A, Fiadotava, A, Tscharke, D et al. 2021, 'Spreading Fun: Comic Zombies, Joker Viruses and COVID-19 Jokes', Journal of Science and Popular Culture, vol. 4, no. 1, pp. 39-57.
- Hochheiser, K, Wiede, F, Wagner, T et al. 2021, 'Ptpn2 and KLRG1 regulate the generation and function of tissue-resident memory CD8+ T cells in skin', Journal of Experimental Medicine, vol. 218, no. 6.
- Lin, C, Croft, S, Croft, N et al. 2021, 'Direct priming of CD8+ T cells persists in the face of cowpox virus inhibitors of antigen presentation', Journal of Virology, vol. 95, no. 10.
- Assmus, L, Guan, J, Wu, T et al. 2020, 'Overlapping peptides elicit distinct CD8+T cell responses following influenza a virus infection', Journal of Immunology, vol. 205, no. 7, pp. 1731-1742.
- Crosse, K, Monson, E, Dumbrepatil, A et al. 2020, 'Viperin binds STING and enhances the type-I interferon response following dsDNA detection', Immunology and Cell Biology, vol. 99, no. 4, pp. 373-391.
- Croft, S, Wong, Y, Smith, S et al. 2020, 'Surprisingly Effective Priming of CD8+ T Cells by Heat-Inactivated Vaccinia Virus Virions', Journal of Virology, vol. 94, no. 20, pp. 1-18.
- Sinu, P, Croft, N, Purcell, A et al. 2020, 'Benchmarking predictions of MHC class I restricted T cell epitopes in a comprehensively studied model system', PLOS Computational Biology, vol. 16, no. 5, pp. -.
- Singh, N & Tscharke, D 2020, 'Herpes Simplex Virus Latency Is Noisier the Closer We Look', Journal of Virology, vol. 94, no. 4, pp. -.
- Gowripalan, A, Smith, S, Stefanovic, T et al. 2020, 'Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool', Communications Biology, vol. 3, no. 1, pp. 1-13.
- Fox, D, Mathur, A, Xue, Y et al. 2020, 'Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome', Nature Communications, vol. 11, no. 760, pp. 1-16.
- Velusamy, T, Gowripalan, A & Tscharke, D 2020, 'CRISPR/Cas9-Based Genome Editing of HSV', in Russell J. Diefenbach, Cornel Fraefel (ed.), Herpes Simplex Virus: Methods and Protocols, Humana New York, New York, pp. 169-183.
- Zanker, D, Oveissi, S, Tscharke, D et al. 2019, 'Influenza A virus infection induces viral and cellular defective ribosomal products encoded by alternative reading frames', Journal of Immunology, vol. 202, no. 12, pp. 3370-3380.
- Wong, Y, Croft, S, Smith, S et al. 2019, 'Modified Vaccinia Virus Ankara Can Induce Optimal CD8(+)T Cell Responses to Directly Primed Antigens Depending on Vaccine Design', Journal of Virology, vol. 93, no. 21.
- Campbell, T, McSharry, B, Steain, M et al. 2019, 'Functional paralysis of human natural killer cells by alphaherpesviruses', PLoS Pathogens, vol. 15, no. 6, pp. e1007784-.
- Croft, N, Smith, S, Pickering, J et al. 2019, 'Most viral peptides displayed by class I MHC on infected cells are immunogenic', PNAS - Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 8, pp. 3112-3117.
- Wu, T, Guan, J, Handel, A et al. 2019, 'Quantification of epitope abundance reveals the effect of direct and cross-presentation on influenza CTL responses', Nature Communications, vol. 10.
- Gebhardt T, Tscharke D, Palendira U, Bedoui S 2018, 'Tissue-resident memory T cells in tissue homeostasis, persistent infection, and cancer surveillance', Immunological Reviews, vol. 283, no. 1, pp. 54-76pp.
- Park SL, Zaid A, Hor JL, Christo SN, Prier JE, Davies B, Alexandre YO, Gregory JL, Russell TA, Gebhardt T, Carbone FR, Tscharke DC, Heath WR, Mueller SN, and Mackay LK. 2018, 'Local proliferation maintains a stable pool of tissue-resident memory T cells after antiviral recall responses article', Nature Immunology, vol. 19, no. 2, pp. 183-191.
- Russell T, Velusamy T, Tseng Y, Tscharke D. 2018, 'Increasing antigen presentation on HSV-1-infected cells increases lesion size but does not alter neural infection or latency', Journal of General Virology, vol. 99, no. 5, pp. 682-692.
- Whitney, P, Makhlouf, C, MacLeod, B et al 2018, 'Effective priming of herpes simplex virus specific CD8+ T cells in vivo does not require infected dendritic cells', Journal of Virology, vol. 92, no. 3, pp. 1-13.
- Westwood, J, Ellis, S, Danne, J et al 2017, 'An ultrastructural investigation of tumors undergoing regression mediated by immunotherapy', Oncotarget, vol. 8, no. 70, pp. 115215-115229pp.
- Slaney, C, von Scheidt, B, Davenport, A et al 2017, 'Dual-specific chimeric antigen receptor T cells and an indirect vaccine eradicate a variety of large solid tumors in an immunocompetent, self-antigen setting', Clinical Cancer Research, vol. 23, no. 10, pp. 2478-2490pp.
- Rezende Melo Da Silva, C, Tscharke, D, Lobigs, M et al 2017, 'Ectromelia virus N1L is essential for virulence but not dissemination in a classical model of mousepox', Virus Research, vol. 228, pp. 61-65.
- Miosge, L, Sontani, Y, Chuah, A, Horikawa, K, Russel, TA, Mei, Y, Wagle, M, Howard, DR, Enders, A, Tscharke, DC, Goodnow, CC and IA Parish. 2017, 'Systems-guided forward genetic screen reveals a critical role of the replication stress response protein ETAA1 in T cell clonal expansion.', PNAS, Online, pp. 1-10pp..
- Purcell, A, Croft, N & Tscharke, D 2016, 'Immunology by numbers: Quantitation of antigen presentation completes the quantitative milieu of systems immunology!', Current Opinion in Immunology, vol. 40, pp. 88-95.
- Russell, T & Tscharke, D 2016, 'Lytic Promoters Express Protein during Herpes Simplex Virus Latency', PLoS Pathogens, vol. 12, no. 6, pp. e1005729-e1005729.
- Gram, A, Oosenbrug, T, Lindenbergh, M et al 2016, 'The Epstein-Barr Virus Glycoprotein gp150 Forms an Immune-Evasive Glycan Shield at the Surface of Infected Cells', PLoS Pathogens, vol. 12, no. 4, pp. e1005550-e1005550.
- Munier, M, Van Bockel, D, Bailey, M et al 2016, 'The primary immune response to Vaccinia virus vaccination includes cells with a distinct cytotoxic effector CD4 T-cell phenotype', Vaccine, vol. 34, no. 44, pp. 5251-5261.
- Croft, N, Purcell, A & Tscharke, D 2015, 'Quantifying epitope presentation using mass spectrometry', Molecular Immunology, vol. 68, no. 2, pp. 77-80.
- Dobson, B & Tscharke, D 2015, 'Redundancy complicates the definition of essential genes for vaccinia virus', Journal of General Virology, vol. 96, no. 11, pp. 3326-3337.
- Russell, T, Stefanovic, T & Tscharke, D 2015, 'Engineering herpes simplex viruses by infection-transfection methods including recombination site targeting by CRISPR/Cas9 nucleases', Journal of Virological Methods, vol. 213, pp. 18-25.
- Croft, N. P., D. A. de Verteuil, S. A. Smith, Y. C. Wong, R. B. Schittenhelm, D. C. Tscharke, and A. W. Purcell. 2015, 'Simultaneous quantification of viral antigen expression kinetics using data-independent (DIA) mass spectrometry', Molecular and Cellular Proteomics, vol. 14, no. 5, pp. 1361-1372.
- Flesch, I. E. A., K. L. Randall, N. A. Hollett, H. Di Law, L. A. Miosge, Y. Sontani, C. C. Goodnow, and D. C. Tscharke. 2015, 'Delayed control of herpes simplex virus infection and impaired CD4+ T-cell migration to the skin in mouse models of DOCK8 deficiency', Immunology and Cell Biology, vol. 93, no. 2015, pp. 517-521.
- Flesch, I., N. Hollett, Y.-C. Wong, B. Quinan, D. Howard, F. da Fonseca, and D. Tscharke. 2015, 'Extent of systemic spread determines CD8+ T cell immunodominance for laboratory strains, smallpox vaccines, and zoonotic isolates of vaccinia virus', Journal of Immunology, vol. 195, no. 5, pp. 2263-2272.
- Tscharke DC, Croft NP, Doherty PC & La Gruta NL. 2015, Sizing up the key determinants of the CD8+ T cell response, Nature Reviews Immunology, vol. 15, no. 11, pp. 705-716.
- Tseng, Y.-Y., F.-Y. Lin, S.-F. Cheng, D. Tscharke, S. Chulakasian, C.-C. Chou, Y.-F. Liu, W.-S. Chang, M.-L. Wong, and W.-L. Hsu. 2015, 'Functional analysis of the short isoform of orf virus protein OV20.0', Journal of Virology, vol. 89, no. 9, pp. 4966-4979.
- Trujillo JA, Croft NP, Dudek NL, Channappanavar R, Theodossis A, Webb AI, Dunstone MA, Illing PT, Butler NS, Fett C, Tscharke DC, Rossjohn J, Perlman S, and Purcell AW 2014, 'The cellular redox environment alters antigen presentation', Journal of Biological Chemistry, vol. 289, no. 40, pp. 27979-27991.
- Ma, J, Russell, T, Spelman, T, *Carbone FR, and *Tscharke DC 2014, 'Lytic Gene Expression Is Frequent in HSV-1 Latent Infection and Correlates with the Engagement of a Cell-Intrinsic Transcriptional Response', PLoS Pathogens, vol. 10, no. 7. e1004237. *Equal contribution
- Russell TA and Tscharke DC 2014, 'Strikingly poor CD8+ T-cell immunogenicity of vaccinia virus strain MVA in BALB/c mice', Immunology and Cell Biology, vol. 92, no. 5, pp. 466-469.
- Macleod BL, Bedoui S, Hor JL, Mueller SN, Russell TA, Hollett NA, Heath WR, Tscharke DC, Brooks AG, and Gebhardt T. 2014, 'Distinct APC Subtypes Drive Spatially Segregated CD4+ and CD8+ T-Cell Effector Activity during Skin Infection with HSV-1', PLoS Pathogens, vol. 10, no. 8. e1004303
- Dobson, B, Procter, D, Hollett NA, Flesch IEA, Newsome TP, and Tscharke DC 2014, 'Vaccinia virus F5 is required fornormal plaque morphology in multiple cell lines but not replication in culture or virulence in mice', Virology, vol. 456-457, pp. 145-156.
- Dobson, B & Tscharke, D 2014, 'Truncation of gene F5L partially masks rescue of vaccinia virus strain MVA growth on mammalian cells by restricting plaque size', Journal of General Virology, vol. 95, pp. 466-471.
- Quinan BR, Flesch IEA, Pinho TMG, Coelho FM, *Tscharke DC, and *da Fonseca FG. 2014, 'An intact signal peptide on dengue virus E protein enhances immunogenicity for CD8+ T cells and antibody when expressed from modified vaccinia Ankara', Vaccine, vol. 32, no. 25, pp. 2972-2979. *Equal contribution
- Mackay LK, Rahimpour A, Ma JZ, Collins N, Stock AT, Hafon M-L, Vega-Ramos J, Lauzurica P, Mueller SN, Stefanovic T, Tscharke DC, Heath WR, Inouye M, Carbone FR, and Gebhardt T. 2013, 'The developmental pathway for CD103+CD8+ tissue-resident memory T cells of skin', Nature Immunology, vol. 14, no. 12, pp. 1294-1301.
- Flesch, I, Wong, Y & Tscharke, D 2012, 'Analyzing CD8 T cells in mouse models of poxvirus infection', in Stuart N. Isaacs (ed.), Vaccinia Virus and Poxvirology. Methods and Protocols, Humana Press, Springer New York, pp. 199-218.
- Kerr PJ, Rogers MB, Fitch A, DePasse JV, Cattadori IM, Twaddle AC, Hudson PJ, Tscharke DC, Read AF, Holmes EC, and Ghedin E. 2013, 'Genome Scale Evolution of Myxoma Virus reveals Host-Pathogen adaptation and rapid Geographic spread', Journal of Virology, vol. 87, no. 23, pp. 12900-12915.
- Wong, Y, Smith, S & Tscharke, D 2013, 'Systemic toll-like receptor ligation and selective killing of dendritic cell subsets fail to dissect priming pathways for anti-vaccinia virus CD8+ T cells', Journal of Virology, vol. 87, no. 22, pp. 11978-11986.
- Kerr P, Rogers M, Fitch A, DePasse J, Cattadori I, Hudson P, Tscharke D, Holmes E, and Ghedin E. 2013, 'Comparative analysis of the complete genome sequence of the california msw strain of myxoma virus reveals potential host adaptations', Journal of Virology, vol. 87, no. 22, pp. 12080-12089.
- Lin, C, Flesch, I & Tscharke, D 2013, 'Immunodomination during Peripheral Vaccinia Virus Infection', PLoS Pathogens, vol. 9, no. 4, pp. e1003329
- Croft NP, Smith SA, Wong YC, Tan CT, Dudek NL, Flesch IEA, Lin LCW, *Tscharke DC, and *Purcell AW. 2013, 'Kinetics of Antigen Expression and Epitope Presentation during Virus Infection', PLoS Pathogens, vol. 9, no. 1, pp. e1003129. *Equal contribution
- Wijesundara, DK, Jackson, RJ, Tscharke, DC and RANASINGHE, C. 2013, 'IL-4 and IL-13 mediated down-regulation of CD8 expression levels can dampen anti-viral CD8+ T cell avidity following HIV-1 recombinant pox viral vaccination', Vaccine, vol. 31, no. 41, pp. 4548-4555.
- Wijesundara, D. K., Tscharke, D. C., Jackson, R. J. and RANASINGHE, C. 2013, 'Reduced Interleukin-4 Receptor alpha Expression on CD8+ T Cells Correlates with Higher Quality Anti-Viral Immunity', PLoS ONE, vol. 8, no. 1, p. e55788.
- Kerr, P, Ghedin, E, DePasse, J et al 2012, 'Evolutionary History and Attenuation of Myxoma Virus on Two Continents', PLoS Pathogens, vol. 8, no. 10, p. e1002950.
- Flesch, I, Wong, Y, Tscharke, D et al 2012, 'Linear fidelity in quantification of anti-viral CD8+ T cells', PLoS ONE, vol. 7, no. 6, p. e39533.
- Lin, C, Smith, S & Tscharke, D 2012, 'An intradermal model for vaccinia virus pathogenesis in mice', in Stuart N. Isaacs (ed.), Vaccinia Virus and Poxvirology. Methods and Protocols, Humana Press, Springer New York, pp. 147-159.
- Wong, Y, Lin, C, Rezende Melo Da Silva, C et al. 2011, 'Engineering recombinant poxviruses using a compact GFP-blasticidin resistance fusion gene for selection', Journal of Virological Methods, vol. 171, no. 1, pp. 295-298.
- Melo-Silva, CR, Tscharke, DC, Lobigs, M, Koskinen, A, Wong, YC, Buller, RM, Mullbacher, M, Regner, M 2011, 'The Ectromelia Virus SPI-2 Protein Causes Lethal Mousepox by preventing NK Cell Responses', Journal of Virology. Vol. 81, No. 21, pp. 11170–11182.
- Flesch, I, Woo, W, Wang, Y et al. 2010, 'Altered CD8+ T cell immunodominance after vaccinia virus infection and the naive repertoire in inbred and F1 mice', Journal of Immunology, vol. 184, no. 1, pp. 45-55.
- Moutaftsi, M, Tscharke, D, Vaughan, K et al 2010, 'Uncovering the interplay between CD8, CD4 and antibody responses to complex pathogens', Future Microbiology, vol. 5, no. 2, pp. 221-239.
- Theodoratos, A., Whittle, B., Enders, A., Tscharke, D. C., Roots, C. M., Goodnow, C. C. & Fahrer, A. M. 2010. 'Mouse strains with point mutations in TAP1 and TAP2', Immunology and Cell Biology, vol. 88, pp. 72-78.
- Yuen, T, Flesch, I, Noel, N et al 2010, 'Analysis of A47, an Immunoprevalent Protein of Vaccinia Virus, leads to a reevaluation of the total antiviral CD8+ T cell response', Journal of Virology, vol. 84, no. 19, pp. 10220-10229.
- Lutzky, V, Davis, J, Crooks, P et al 2009, 'Optimization of LMP-specific CTL expansion for potential adoptive immunotherapy in NPC patients', Immunology and Cell Biology, vol. 87, pp. 481-488.
- Wang, Y, Flesch, I & Tscharke, D 2009, 'Vaccinia Virus CD8+ T-Cell Dominance Hierarchies Cannot Be Altered by Prior Immunization with Individual Peptides', Journal of Virology, vol. 83, no. 17, pp. 9008-9012.
- Sette, A, Grey, H, Oseroff, C et al 2009, 'Definition of epitopes and antigens recognized by vaccinia specific immune responses: Their conservation in variola virus sequences, and use as a model system to study complex pathogens', Vaccine, vol. 27S, pp. G21-G26.
- Assarsson, E, Greenbaum, J, Sundstrom, M et al 2008, 'Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes', PNAS - Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 6, pp. 2140-2145.
- Tellam, J, Smith, C, Rist, M et al 2008, 'Regulation of protein translation through mRNAstructure influences MHC class I loading andT cell recognition', PNAS - Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 27, pp. 9319-9324.
- Oseroff, C, Peters, B, Pasquetto, V et al. 2008, 'Dissociation between epitope hierarchy and immunoprevalence in CD8 responses to vaccinia virus Western Reserve', Journal of Immunology, vol. 180, no. 11, pp. 7193-7202.
- Haeryfar, S, Hickman, H, Irvine, K et al 2008, 'Terminal Deoxynucleotidyl Transferase Establishes and Broadens Antiviral CD8+ T Cell Immunodominance Hierarchies', Journal of Immunology, vol. 181, pp. 649-659.
- Fischer, M, Tscharke, D, Donohue, K et al 2007, 'Reduction of vector gene expression increases foreign antigen-specific CD8+ T-cell priming', Journal of General Virology, vol. 88, pp. 2378-2386.
- Moor, R, Morrison, L, Moss, D et al 2007, 'Use of CD107-based cell sorting ex vivo to enrich subdominant CD8+ T cells in culture', Immunology and Cell Biology, vol. 85, pp. 546-550.
- Tellam, J, Fogg, M, Rist, M et al 2007, 'Influence of translation efficiency of homologous viral proteins on the endogenous presentation of CD8+ T cell epitopes', Journal of Experimental Medicine, vol. 204, no. 3, pp. 525-532.
- Tscharke, D 2007, 'Adaptive immunity to vaccinia virus: revisiting an old friend', Future Virology, vol. 2, no. 2, pp. 163-172.
- Clark, R, Kenyon, J, Bartlett, N et al 2006, 'Deletion of gene A41L enhances vaccinia virus immunogenicity and vaccine efficacy', Journal of General Virology, vol. 87, pp. 29-38.
- Moutaftsi, M, Peters, B, Pasquetto, V et al 2006, 'A consensus epitope prediction approach identifies the breadth of murine TCD8+-cell responses to vaccinia virus', Nature Biotechnology, vol. 24, no. 7, pp. 817-19.
- Tscharke, D, Woo, W, Sakala, I et al 2006, 'Poxvirus CD8+ T-cell determinants and cross-reactivity in BALB/c mice', Journal of Virology, vol. 80, pp. 6318-23.
- Haeryfar, S, DiPaolo, R, Tscharke, D et al 2005, 'Regulatory T cells suppress CD8+ T cell responses induced by direct priming and cross-priming and moderate immunodominance disparities', Journal of Immunology, vol. 174, no. 6, pp. 3344-51.
- Pasquetto, V, Bui, H, Giannino, R et al. 2005, 'HLA-A*0201, HLA-A*1101, and HLA-B*0702 transgenic mice recognize numerous poxvirus determinants from a wide variety of viral gene products', Journal of Immunology, vol. 175, no. 8, pp. 5504-15.
- Tscharke, D & Suhrbier, A 2005, 'From mice to humans. Murine intelligence for human CD8+ T cell vaccine design', Expert Opinion on Biological Therapy, vol. 5, no. 2, pp. 263-71.
- Tscharke, D, Karupiah, G, Zhou, J et al 2005, 'Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines', Journal of Experimental Medicine, vol. 201, no. 1, pp. 95-104.
- Oseroff, C, Kos, F, Bui, H et al 2005, 'HLA class I-restricted responses to vaccinia recognize a broad array of proteins mainly involved in virulence and viral gene regulation', PNAS - Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 39, pp. 13980-13985.
- Smith, C, Mirza, F, Pasquetto, V et al 2005, 'Immunodominance of poxviral-specific CTL in a human trial of recombinant-modified vaccinia Ankara', Journal of Immunology, vol. 175, no. 10, pp. 8431-37.
- Norbury, C, Basta, S, Donohue, K et al 2004, 'CD8+ T Cell Cross-Priming via Transfer of Proteasome Substrates', Science, vol. 304, pp. 1318-1321.
- Tscharke, D & Yewdell, J 2003, 'T cells bite the hand that feeds them (News and Views)', Nature Medicine, vol. 9, pp. 647-48.
- Pires de Miranda, M, Reading, P, Tscharke, D et al 2002, 'The vaccinia virus kelch-like protein C2L affects calcium-independent adhesion to the extracellular matrix and inflammation in a murine intradermal model', Journal of General Virology, vol. 84, pp. 2459-71.
- Symons, J, Adams, E, Tscharke, D et al 2002, 'The vaccinia virus C12L protein inhibits mouse IL-18 and promotes virus virulence in the murine intranasal model', Journal of General Virology, vol. 83, pp. 2833-2844.
- Tscharke, D, Reading, P & Smith, G 2002, 'Dermal infection with vaccinia virus reveals roles for virus proteins not seen using other inoculation routes', Journal of General Virology, vol. 83, pp. 1977-86.
- Price, N, Tscharke, D & Smith, G 2002, 'The vaccinia virus B9R protein is a 6 kDa intracellular protein that is non-essential for virus replication and virulence', Journal of General Virology, vol. 83, pp. 873-878.
- Symons, J, Tscharke, D, Price, N et al 2002, 'A study of the vaccinia virus interferon-y receptor and its contribution to virus virulence', Journal of General Virology, vol. 83, pp. 1953-64.
- Bartlett, N, Symons, J, Tscharke, D et al 2002, 'The vaccinia virus N1L protein is an intracellular homodimer that promotes virulence', Journal of General Virology, vol. 83, pp. 1965-1976.
- Tscharke, D & Smith, G 2002, 'Notes on transient host range selection for engineering vaccinia virus strain MVA', BioTechniques, vol. 33, pp. 186-8.
- Yewdell, J & Tscharke, D 2002, 'Inside the professionals (News and Views)', BioTechniques, vol. 418, pp. 923-4.
- Gardner, J, Tscharke, D, Reading, P et al 2001, 'Vaccinia virus semaphorin A39R is a 50-55 kDa secreted glycoprotein that affects the outcome of infection in a murine intradermal model', Journal of General Virology, vol. 82, pp. 2083-2093.
- Ng, A, Tscharke, D, Reading, P et al 2001, 'The vaccinia virus A41L protein is a soluble 30 kDa glycoprotein that affects virus virulence', Journal of General Virology, vol. 82, pp. 2095-2105.
- Almazan, F, Tscharke, D & Smith, G 2001, 'The vaccinia virus superoxide dismutase-like protein (A45R) is a virion component that is nonessential for virus replication', Journal of Virology, vol. 75, pp. 7018-7029.
Projects and Grants
Grants information is drawn from ARIES. To add or update Projects or Grants information please contact your College Research Office.
- RNA-binding proteins rewire transcriptomes in immune cell differentiation (Secondary Investigator)
- Reducing the burden of DNA viruses on human health (Primary Investigator)
- NHMRC 2021 Equipment Grant - Exoid (Izon Biosciences) - Linked to ARIES ID 39296 (Secondary Investigator)
- Targeting miRNA biogenesis to treat herpes simplex virus latency (Primary Investigator)
- Strategies to prevent two viruses devaluing Australian crocodile skins (Secondary Investigator)
- Understanding the role of ongoing viral activity in herpes simplex virus latency (Primary Investigator)
- GentleMACS Octo Tissue Dissociator (See 32537) (Primary Investigator)
- Understanding how viral innate immune evasion strategies affect adaptive immunity, and the application to vaccine development (Secondary Investigator)
- A proteome-wide approach to anti-viral immunity and vaccine development (Primary Investigator)
- A humanised mouse model for herpes simplex virus pathogenesis (Primary Investigator)
- Recognition of virus-infected cells by T cells (Primary Investigator)
- A new viral vaccine platform (Primary Investigator)
- Quantification of antigen presentation to CD8+T cells during virus infection (Primary Investigator)
- Virus and host genes and the outcome of infection (Primary Investigator)
- The role of virus gene expression and CD8+ T cell immunity in latency and reactivation of herpes simplex virus (Primary Investigator)
- CD8* T Cell immunity to viruses and recombinant vaccines (Primary Investigator)