Emeritus Professor Christopher Parish
B Agr Sci (U Melb), PhD (U Melb)
ANU College of Health and Medicine
T:
6125 2604
Areas of expertise
- Medical Biochemistry: Carbohydrates 110102
- Cellular Interactions (Incl. Adhesion, Matrix, Cell Wall) 060106
- Cellular Immunology 110704
- Autoimmunity 110703
- Tumour Immunology 110709
- Immunology 1107
Biography
Professor Chris Parish received his undergraduate training at the University of Melbourne and then gained a PhD degree in immunology at The Walter & Eliza Hall Institute, Melbourne. He is currently Leader of the Cancer and Vascular Biology Group, Department of Immunology, John Curtin School of Medical Research, ANU. During the last 35 years he has investigated various aspects of the immune system. During the early 1970's he was the first person to demonstrate that cell-mediated immunity and antibody formation are mutually antagonistic responses, an observation that underpins the Th1/Th2 paradigm in immunology. He is currently attempting to harness immune deviation to produce novel anti-cancer vaccines. Since the late 1970's Professor Parish has also been interested in the role that complex carbohydrates, particularly heparan sulfate, play in cell adhesion and cell migration. These studies have resulted in heparanase, the enzyme that degrades heparan sulfate, being identified as a drug target and has formed the theoretical basis for the development of several carbohydrate-based drugs, such as PI-88 (Muparfostat), that inhibit inflammation, tumour metastasis and angiogenesis. In addition, Professor Parish’s laboratory has developed a number of important immunological techniques, such as fluorescent dyes (e.g., CFSE) for monitoring lymphocyte migration and proliferation. His research findings underpin several Australian biotechnology companies, such as Progen Pharmaceuticals and Lipotek. In 2005 Prof Parish was awarded the Clive and Vera Ramaciotti Medal for Excellence in Biomedical Research in recognition of his scientific achievements.
Researcher's projects
The Cancer and Vascular Biology Group, led by Prof Parish, is generally interested in cell biology aspects of immune regulation, tumour metastasis, angiogenesis and inflammation. More specific research areas are:
(1) Investigating our remarkable finding that both B and T lymphocytes, when activated, can transfer their antigen receptors to bystander lymphocytes by membrane exchange. This process enhances the number of B cells that can capture and present antigen to CD4+ T cells and dramatically expands the number of CD8+ cytotoxic T cells that can clear a viral infection. However, the molecular basis of this phenomenon and its importance in various immune responses needs to be clarified and is an area of intense investigation.
(2) Harnessing the immune response against cancer. We have shown that tumour-specific CD4+ T cells, with a Th2 cytokine profile, are extremely effective at eliminating tumours by recruiting tumouricidal eosinophils into tumours. Eosinophils also play a critical role in tumour immunosurveillance. How eosinophils eliminate tumours and whether adaptive immunity is essential for eosinophil activation are areas of current research. In collaboration with Dr Joe Altin (Research School of Biology, ANU) we have also developed a liposome-based vaccine against cancer that targets dendritic cells. Research is continuing, in collaboration with the start-up biotechnology company Lipotek, to develop better liposome-based vaccines against cancer and other diseases.
(3) Investigating the role of heparan sulfate and heparanase in tumour metastasis, angiogenesis and inflammation is a broad area of research in which the Prof Parish's Laboratory has participated for over 25 years. This area of research is continuing. Interests include the role of heparan sulfate and heparanase in the development of Type I diabetes, the ability of heparanase to localise to the nucleus of cells and regulate gene expression and the development of heparan sulfate mimetics as anti-inflammatory agents.
(4) For many years Prof Parish's Laboratory has been studying histidine-rich glycoprotein (HRG), a blood borne protein that binds heparan sulfate and many other ligands and has been implicated in tumour metastasis and angiogenesis. Research on this molecule continues, with our recent studies suggesting that HRG may play an important role in controlling autoimmune diseases, such as systemic lupus, by aiding the elimination of dead and dying cells.
(5) A relatively new area of research is investigating the role of platelets in tumour metastasis. Our recent studies suggest that platelets can influence tumour metastasis at two levels, namely (i) by adhering to tumour cells within a tumour, inducing the expression of many genes involved in cell migration and allowing tumour cells to escape from the primary tumour and (ii) by cross-linking blood borne tumour cells to the endothelium and thereby aiding the subsequent entry of tumour cells into tissues. Validation of this hypothesis is a very active area of research.
(6) Finally the Laboratory continues to use in vitro angiogenesis assays, developed in-house, to test compounds from other researchers that may have pro- or anti-angiogenic activities. Pro-angiogenic compounds represent potential therapeutics for cardiovascular disease and anti-angiogenic compounds could be used as anti-cancer agents. The most successful of these collaborations has been with the ARC Centre for Integrative Legume Research where legume associated nodulation factors, which are modified chito-oligosaccharides, have been shown, depending on their structure, to be either pro- or anti-angiogenic.
Publications
- Dhounchak, S, Popp, S, Brown, D et al. 2021, 'Heparan sulfate proteoglycans in beta cells provide a critical link between endoplasmic reticulum stress, oxidative stress and type 2 diabetes', PLOS ONE (Public Library of Science), vol. 16, no. 6.
- Yoon, J, Parish, C, Blackburn, A et al. 2021, 'Potential contrasting effects of platelets on the migration and invasion of sarcomas versus carcinomas', Platelets, vol. 32, no. 5, pp. 662-670.
- Khanna, M & Parish, C 2020, 'Heparanase: Historical Aspects and Future Perspectives', in I Vlodavsky, R D Sanderson, N Ilan (ed.), Heparanase, Springer, Switzerland, pp. 71-96.
- Simeonovic, C, Popp, S, Brown, D et al. 2020, 'Heparanase and Type 1 Diabetes', in I Vlodavsky, R D Sanderson, N Ilan (ed.), Heparanase, Springer, Switzerland, pp. 607-630.
- Yoon, J, Parish, C & Coupland, L 2020, 'A Rapid and Accurate Bioluminescence-Based Migration Assay Permitting Analysis of Tumor Cell/Stromal Cell Interactions', Methods and Protocols, vol. 3, no. 10, pp. 1-12.
- Montague PhD, S, Hicks, S, Lee, C et al. 2020, 'Fibrin exposure triggers αIIbβ3-independent platelet aggregate formation, ADAM10 activity and glycoprotein VI shedding in a charge-dependent manner', Journal of Thrombosis and Haemostasis, vol. 18, no. 6, pp. 1447-1458.
- O'Meara, C, Coupland, L, Kordbacheh, F et al. 2020, 'Neutralizing the pathological effects of extracellular histones with small polyanions', Nature Communications, vol. 11, no. 1.
- Khanna, M, Ranasinghe, C, Browne, A et al 2019, 'Is host heparanase required for the rapid spread of heparan sulfate binding viruses?', Virology, vol. 529, pp. 1-6.
- Simeonovic, C, Popp, S, Starrs, L et al 2018, 'Loss of intra-islet heparan sulfate is a highly sensitive marker of type 1 diabetes progression in humans ', PLOS ONE (Public Library of Science), vol. 13, no. 2: e0191360, pp. 1-18.
- Kordbacheh, F, Carruthers, T, Orlov, A et al 2018, 'Promotion of mammalian angiogenesis by neolignans derived from soybean extracellular fluids', PLOS ONE (Public Library of Science), vol. 13, no. 5, pp. 21pp.
- Sheahan, A, Biankin, A, Parish, C et al 2018, 'Targeted therapies in the management of locally advanced and metastatic pancreatic cancer: A systematic review', Oncotarget, vol. 9, no. 30, pp. 21613-21627.
- Cai, H, Cho, E, Li, Y et al 2018, 'Melanoma protective anti-tumor immunity activated by catalytic DNA', Oncogene, vol. 37, pp. 5115-5126pp.
- Gargett, T, Abbas, M, Rolan, P et al 2018, 'Phase I trial of Lipovaxin-MM, a novel dendritic cell-targeted liposomal vaccine for malignant melanoma', Cancer Immunology, Immunotherapy, vol. 67, pp. 1461-1472pp.
- Simeonovic, C, Popp, S, Starrs, L et al 2017, 'Loss of intra-islet heparan sulfate represents a novel marker for the progression of type 1 diabetes in humans', 53rd EASD Annual Meeting, Springer, Germany, pp. S178-S179.
- Lee, E, Dykas, D, Leavitt, A et al 2017, 'Whole-exome sequencing in evaluation of patients with venous thromboembolism', Blood Advances, vol. 1, no. 16, pp. 1224-1237pp.
- Peterson, E, Daniel, G, Katner, S et al 2017, 'Antiangiogenic platinum through glycan targeting', Chemical Science, vol. 8, no. 1, pp. 241-252.
- Kordbacheh, F, O'Meara, C, Coupland, L et al 2017, 'Extracellular histones induce erythrocyte fragility and anemia', Blood, vol. 130, no. 26, pp. 2884-2888.
- Buckler, J, Banwell, M, Kordbacheh, F et al 2017, 'Developing neolignans as proangiogenic agents: Stereoselective total syntheses and preliminary biological evaluations of the four guaiacylglycerol 8-O-4'-coniferyl ethers', ACS Omega, vol. 2, no. 10, pp. 7375-7388.
- Coupland, L, Hindmarsh, L, Gardiner, E et al 2017, 'The influence of platelet membranes on tumour cell behaviour', Cancer and Metastasis Reviews, vol. 36: 215-224
- Khanna M, RANASINGHE C, Jackson R, Parish CR. 2017 'Heparan sulfate as a receptor for poxvirus infections and as a target for antiviral agents', Journal of General Virology, vol. 98, no. 10, pp. 2556-2568.
- Mishra, AK, Parish, CR, Wong, ML, Licinio J, Blackburn AC 2017, 'Leptin signals via TGFB1 to promote metastatic potential and stemness in breast cancer', PLOS ONE (Public Library of Science), vol. 12, no. 5, pp. 1-21pp.
- Sinha, D, Chong, L, George, J et al 2016, 'Pericytes promote malignant ovarian cancer progression in mice and predict poor prognosis in serous ovarian cancer patients', Clinical Cancer Research, vol. 22, no. 7, pp. 1813-1824.
- Kirk, N, Orlov, A, Willis, A et al 2016, 'Synthesis and preliminary evaluation of 5,7-dimethyl-2-aryl-3H-pyrrolizin-3-ones as angiogenesis inhibitors', Bioorganic and Medicinal Chemistry Letters, vol. 26, no. 7, pp. 1813-1816pp.
- Stephens, RW, Parish, CR, Freeman, CG, Senden, TJ 2016, Histone Inhibition. US Patent 9,226,939
- Nossal, G & Parish, C 2015, 'Gordon Leslie Ada 1922-2012', Historical Records of Australian Science, vol. 26, no. 2, pp. 164-178.
- Quah, B & Parish, C 2015, 'Innate Immune Mechanisms: Nonself recognition', in Wiley (ed.), Encylopedia of Life Sciences (2015), John Wiley & Sons Inc, UK, pp. 1-11pp.
- Fraser, S, Midwinter, R, Coupland, L et al 2015, 'Heme oxygenase-1 deficiency alters erythroblastic Island formation, steady-state erythropoiesis and red blood cell lifespan in mice', Haematologica, vol. 100, no. 5, pp. 601-610.
- Coombe, D & Parish, C, eds, 2015, Carbohydrates: The yet to be tasted sweet spot of immunity, Frontiers Media, Lausanne.
- Coombe, D & Parish, C 2015, 'Editorial: Carbohydrates: the yet to be tasted sweet spot of immunity', Frontiers in Immunology, vol. 6, pp. 314-314.
- Choong, F, Freeman, C, Parish, C, Simeonovic CJ 2015, 'Islet Heparan Sulfate but Not Heparan Sulfate Proteoglycan Core Protein Is Lost During Islet Isolation and Undergoes Recovery Post-Islet Transplantation', American Journal of Transplantation, vol. 15, pp. 2861-2864.
- Pang, J, Coupland, L, Freeman, C, Chong, BH and Parish CR. 2015, 'Activation of tumour cell ECM degradation by thrombin-activated platelet membranes: potentially a P-selectin and GPIIb/IIIa-dependent process', Clinical and Experimental Metastasis, vol. 32, no. 5, pp. 495-505.
- Stephens, RW, Knox, KJ, Philip, LA et al 2015, 'The uptake of soluble and nanoparticulate imaging isotope in model liver tumours after intra-venous and intra-arterial administration', Biomaterials, vol. 39, pp. 218-224.
- Dunn, L, Midwinter, R, Ni, J et al 2014, 'New Insights into Intracellular Locations and Functions of Heme Oxygenase-1', Antioxidants and Redox Signaling, vol. 20, no. 11, pp. 1723-1742.
- Coupland, L, Chong, B & Parish, C 2014, 'Platelets, selectins, and the control of tumor metastasis', Seminars in Oncology, vol. 41, no. 3, pp. 422-434.
- Djordjevic, M, Orlov, A, Susanti, . et al 2014, 'Lipo-chitin oligosaccharides, plant symbiosis signalling molecules that modulate mammalian angiogenesis in vitro', PLOS ONE (Public Library of Science), vol. 9, no. 12, pp. e112635-e112635.
- Parish, C 2014, 'Immunology and cell biology turns 90', Immunology and Cell Biology, vol. 92, no. 1, pp. 1-5.
- Irving-Rodgers, H, Choong, F, Hummitzsch, K et al 2014, 'Pancreatic Islet basement membrane loss and remodeling after mouse islet isolation and transplantation: Impact for allograft rejection', Cell Transplantation, vol. 23, no. 1, pp. 59-72.
- DeRose R., S. J. Kent and RANASINGHE, C (2014). Prime-boost vaccination: Impact on the HIV vaccine field. Novel approaches and satrategies for biologics, vaccines and cancer therapies, Elsevier publication edited by Drs. Manmohan Singh and Maya Salnikova. 2014
- Quah, B, Wijesundara, D, RANASINGHE, C et al 2014, 'The use of fluorescent target arrays for assessment of T cell responses in vivo', Journal of Visualized Experiments, no. 88, pp. e51627-.
- Yabas, M, Coupland, L.A., Cromer, D., Winterberg, M., Teoh, N.C. D'Rozario, J., Kirk, K., Bröer, S., Paris, C.R. and A. Enders 2014, 'Mice deficient in the putative phospholipid flippase ATP11C exhibit altered erythrocyte shape, anemia, and reduced erythrocyte life span', Journal of Biological Chemistry, 289, 19531-19537.
- Simeonovic, C & Parish, C 2013, 'Comment on: Korpos et al. The peri-islet basement membrane, a barrier to infiltrating leukocytes in type 1 diabetes in mouse and human. Diabetes 2013;62:531-542', Diabetes, vol. 62, no. 8, pp. e13-e13.
- Bogdani, M, Korpos, E, Simeonovic, C et al 2012, 'Extracellular Matrix Components in the Pathogenesis of Type 1 Diabetes', Current Diabetes Reports, vol. 14, no. 12, pp. 552-552.
- Khachigian, L, Cai, H, Moloney, F et al 2012, 'Destroying c-jun messenger: New insights into biological mechanisms of DNAzyme Function', Oncotarget, vol. 3, no. 6, pp. 594-595.
- Armitt, D, Banwell, M, Freeman, C et al 2002, 'C-glycoside formation via Lewis acid promoted reaction of O-glycosylimidates with pyrroles', Journal of the Chemical Society, Perkin Transactions 1, vol. 2002, no. 15, pp. 1743-1745.
- Sudta, P, Kirk, N, Orlov, A et al 2013, 'Synthesis, structural characterisation, and preliminary evaluation of non-indolin-2-one-based angiogenesis inhibitors related to sunitinib (Sutent®)', Australian Journal of Chemistry, vol. 66, no. 8, pp. 864-873.
- Coupland, L, Chong, B & Parish, C 2013, 'Beware of NK cells in pre-clinical metastasis models', Clinical and Experimental Metastasis, vol. 30, no. 7, pp. 945-947.
- Simon Davis, D & Parish, C 2013, 'Heparan sulfate: a ubiquitous glycosaminoglycan with multiple roles in immunity', Frontiers in Immunology, vol. 4, pp. 1-7.
- Freeman, CG, Parish, CR, Knox, KJ, Blackmore JL, Lobov SA, King DW, Senden TJ, Stephens RW. 2013, 'The accumulation of circulating histones on heparan sulphate in the capillary glycocalyx of the lungs', Biomaterials, vol. 34, no. 22, pp. 5670-5676.
- Parish, C, Freeman, C, Ziolkowski, A, He YQ, Sutcliffe EL, Zafar A, Rao S, Simeonovic CJ. 2013, 'Unexpected new roles for heparanase in Type 1 diabetes and immune gene regulation', Matrix Biology, vol. 32, no. 5, pp. 228-233.
- Quah, B, Wijesundara, D, RANASINGHE, C, Parish, CR 2013, 'Fluorescent target array T helper assay: A multiplex flow cytometry assay to measure antigen-specific CD4+ T cell-mediated B cell help in vivo', Journal of Immunological Methods, vol. 387, no. 1-2, pp. 181-190.
- Simeonovic, C, Ziolkowski, A, Wu, Z, Choong FJ, Freeman C, Parish CR. 2013, 'Heparanase and autoimmune diabetes', Frontiers in Immunology, vol. 4, pp. 1-7.
- Watson, C, Vine, K, Locke, J et al 2013, 'The antiangiogenic properties of sulfated beta-cyclodextrins in anticancer formulations incorporating 5-fluorouracil', Anti-Cancer Drugs, vol. 24, no. 7, pp. 704-714.
- Quah, B & Parish, C 2012, 'New and improved methods for measuring lymphocyte proliferation in vitro and in vivo using CFSE-like fluorescent dyes', Journal of Immunological Methods, vol. 379, no. 1-2, pp. 1-14.
- Coupland, L, Chong, B & Parish, C 2012, 'Platelets and P-selectin control tumor cell metastasis in an organ-specific manner and independently of NK cells', Cancer Research, vol. 72, no. 18, pp. 4662-4671.
- Yuan, L, Hu, J, Luo, Y et al 2012, 'Upregulation of heparanase in high-glucose-treated endothelial cells promotes endothelial cell migration and proliferation and correlates with Akt and extracellular-signal-regulated kinase phosphorylation', Molecular Vision, vol. 18, no. 169-73, pp. 1684-1695.
- Quah, B, Lyons, A & Parish, C 2012, 'The use of CFSE-like dyes for measuring lymphocyte proliferation: Experimental considerations and biological variables', Mathematical Modelling of Natural Phenomena, vol. 7, no. 5, pp. 53-64.
- Hu, J, Song, X, He, Y et al 2012, 'Heparanase and vascular endothelial growth factor expression is increased in hypoxia-induced retinal neovascularization', Investigative Ophthalmology and Visual Science, vol. 53, no. 11, pp. 6810-6817.
- Cai, H, Santiago, F, Prado-Lourenco, L et al 2012, 'DNAzyme Targeting c-jun Suppresses Skin Cancer Growth', Science Translational Medicine, vol. 4, no. 139, pp. 139ra82-139ra82.
- Ishii, H, Hulett, M, Li, J et al 2012, 'Yin Yang-1 inhibits tumor cell growth and inhibits p21(WAF1/Cip1) complex formation with Cdk4 and cyclin D1', International Journal of Oncology, vol. 40, no. 5, pp. 1575-1580.
- Ahamed MB, Aisha AF, Nassar ZD, Siddiqui JM, Ismail Z, Omari SM, Parish CR, Majid AMl 2012, 'Cat's whiskers tea (orthosiphon stamineus) extract inhibits growth of colon tumor in nude mice and angiogenesis in endothelial cells via suppressing VEGFR phosphorylation', Nutrition and Cancer, vol. 64, no. 1, pp. 89-99.
- Chan, CWS, Kaplan, W, Parish, CR, Khachigian, LM 2012, 'Reduced retinal microvascular density, improved forepaw reach, comparative microarray and gene set enrichment analysis with c-jun targeting DNA enzyme', PLOS ONE (Public Library of Science), vol. 7, no. 7, e39160.
- Hamid M.A., Jackson R.J., Roy S., Khanna M, and RANASINGHE C. 2018 Unexpected involvement of IL-13 signalling via a STAT6 independent mechanism, during IgG2a development Following viral vaccination. European Journal of Immunology Jul;48(7):1153-1163.
- He YQ, Sutcliffe EL, Bunting KL, Li J, Goodall KJ, Poon IK, Hulett MD, Freeman C, Zafar A, McInnes RL, Taya T, Parish CR, Rao S. 2012 The endoglycosidase heparanase enters the nucleus of T lymphocytes and modulates H3 methylation at actively transcribed genes via the interplay with key chromatin modifying enzymes. Transcription. vol 3, pp130-145.
- Ziolkowski AF, Popp SK, Freeman C, Parish CR, Simeonovic CJ. 2012, 'Heparan sulfate and heparanase play key roles in mouse β cell survival and autoimmune diabetes', Journal of Clinical Investigation, vol. 122, pp. 132-141.
- Gandhi NS, Freeman C, Parish CR, Mancera RL. 2012, 'Computational analyses of the catalytic and heparin-binding sites and their interactions with glycosaminoglycans in glycoside hydrolase family 79 endo-Beta-D-glucuronidase (heparanase)', Glycobiology, vol. 22, pp. 35-55.
- Hu J, Song X, He YQ, Freeman C, Parish CR, Yuan L, Yu H, Tang S. 2012. Heparanase and vascular endothelial growth factor expression is increased in hypoxia-induced retinal neovascularization. Invest Ophthalmol Vis Sci. vol 53 pp. 6810-6817.
- Ishii, H., Hulett, M.D., Li, J.M., Santiago, F.S., Parish, C.R. and Khachigian, L.M. (2012). Yin yang-1 inhibits tumor cell growth and inhibits p21WAF1/Cip1 complex formation with cdk4 and cyclin D1. International Journal of Oncology 40, pp1575-1580.
- Abdus Salam Abul, F, Herringson, T, Parish, C et al 2011, 'pDNA-lipoplexes engrafted with flagellin-related peptide induce potent immunity and anti-tumour effects', Vaccine, vol. 29, no. 40, pp. 6911-6919.
- Collison, E, Wimmer-Kleikamp, S, Gerega, S et al 2011, 'The yeast homolog of heme oxygenase-1 affords cellular antioxidant protection via the transcriptional regulation of known antioxidant genes', Journal of Biological Chemistry, vol. 286, no. 3, pp. 2205-2214.
- Poon, I, Patel, K, Simon Davis, D et al 2011, 'Histidine-rich glycoprotein: The Swiss Army knife of mammalian plasma', Blood, vol. 117, no. 7, pp. 2093-2101.
- Shengule, S, Loa-Kum-Cheung, W, Parish, C et al 2011, 'A one-pot synthesis and biological activity of ageladine A and analogues', Journal of Medicinal Chemistry, vol. 54, no. 7, pp. 2492-2503.
- Chen, J, Liu, M, Parish, C et al 2011, 'Nuclear import of early growth response-1 involves importin-7 and the novel nuclear localization signal serine-proline-serine', The International Journal of Biochemistry and Cell Biology, vol. 43, no. 6, pp. 905-912.
- Sutcliffe, E., Bunting, K.L., Yi, Q.H., Li, J., Zafar, A., Hindmarsh, E., Parish, C.R., McInnes, R.L., Taya, T., Milburn, P.J. and Rao, S 2011, 'Chromatin-Associated Protein Kinase C-θ Regulates an Inducible Gene Expression Program and MicroRNAs in Human T Lymphocytes', Molecular Cell, vol. 41, no. 6, pp. 704-719.
- Quah, B & Parish, C 2010, 'Innate Immune Mechanisms: Nonself recognition', in Hildegard Kehrer-Sawatzki (ed.), Encyclopedia of Life Sciences (2010), John Wiley & Sons Inc, Chichester, p. 10.
- Simson, L, Ellyard, J & Parish, C 2010, 'The Role of Th2-Mediated Anti-Tumor Immunity in Tumor Surveillance and Clearance', in M. Penichet, E. Jensen-Jarolim (ed.), Cancer and IgE: Introducing the concept of AllergoOncology, Springer, New York, pp. 255-275.
- Quah, B & Parish, C 2010, 'The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation.', Journal of Visualized Experiments, no. 44, pp. 1-3.
- Coupland, L, Cromer, D, Davenport, M et al 2010, 'A novel fluorescent-based assay reveals that thrombopoietin signaling and Bcl-XL influence, respectively, platelet and erythrocyte lifespans', Experimental Hematology, vol. 38, no. 6, pp. 453-461.
- Poon, I, Parish, C & Hulett, M 2010, 'Histidine-rich glycoprotein functions cooperatively with cell surface heparan sulfate on phagocytes to promote necrotic cell uptake', Journal of Leukocyte Biology, vol. 88, no. 3, pp. 559-569.
- Liang, S, Pinkevych, M, Khachigian, L et al 2010, 'Drug-induced thrombocytopenia: Development of a novel NOD/SCID mouse model to evaluate clearance of circulating platelets by drug-dependent antibodies and the efficacy of IVIG', Blood, vol. 116, no. 11, pp. 1958-1960.
- Poon, I, Hulett, M & Parish, C 2010, 'Histidine-rich glycoprotein is a novel plasma pattern recognition molecule that recruits IgG to facilitate necrotic cell clearance via FcγRI on phagocytes', Blood, vol. 115, no. 12, pp. 2473-2482.
- Poon, I, Hulett, M & Parish, C 2010, 'Molecular mechanisms of late apoptotic/necrotic cell clearance', Cell Death and Differentiation, vol. 17, no. 3, pp. 381-397.
- Coupland, L.A., Cromer, D., Davenport, M.P. & Parish, C.R. A novel fluorescent-based assay reveals that thrombopoietin signaling and Bcl-X(L) influence, respectively, platelet and erythrocyte lifespans. Exp Hematol 38, 453-461 (2010).
- Ellyard, J, Quah, B, Simson, L et al 2010, 'Alternatively activated macrophage possess antitumor cytotoxicity that is induced by IL-4 and mediated by arginase-1', Journal of Immunotherapy, vol. 33, no. 5, pp. 443-452.
- Ellyard, J.I., Quah, B.J., Simson, L. & Parish, C.R. Alternatively activated macrophage possess antitumor cytotoxicity that is induced by IL-4 and mediated by arginase-1. J Immunother 33, 443-452 (2010).
- Klaver DW, Wilce MC, Gasperini R, Freeman C, Juliano JP, Parish C, Foa L, Aguilar MI, Small DH. 2010, 'Glycosaminoglycan-induced activation of the β-secretase (BACE1) of Alzheimer's disease', Journal of Neurochemistry, vol. 112, no. 6, pp. 1552-1561.
- Klaver, D.W., et al. Glycosaminoglycan-induced activation of the beta-secretase (BACE1) of Alzheimer's disease. J Neurochem 112, 1552-1561 (2010)
- Liang, S.X., et al. Drug-induced thrombocytopenia: development of a novel NOD/SCID mouse model to evaluate clearance of circulating platelets by drug-dependent antibodies and the efficacy of IVIG. Blood 116, 1958-1960 (2010).
- Poon IK, Yee DY, Jones AL, Wood RJ, Davis DS, Freeman C, Parish CR, Hulett MD. 2010, 'Histidine-rich glycoprotein binds heparanase and regulates its enzymatic activity and cell surface interactions', The International Journal of Biochemistry and Cell Biology, vol. 42, no. 9, pp. 1507-1516.
- Poon, I.K., et al. Histidine-rich glycoprotein binds heparanase and regulates its enzymatic activity and cell surface interactions. Int J Biochem Cell Biol 42, 1507-1516 (2010).
- Poon, I.K., Hulett, M.D. & Parish, C.R. Histidine-rich glycoprotein is a novel plasma pattern recognition molecule that recruits IgG to facilitate necrotic cell clearance via FcgammaRI on phagocytes. Blood 115, 2473-2482 (2010).
- Poon, I.K., Hulett, M.D. & Parish, C.R. Molecular mechanisms of late apoptotic/necrotic cell clearance. Cell Death Differ 17, 381-397 (2010).
- Poon, I.K., Parish, C.R. & Hulett, M.D. Histidine-rich glycoprotein functions cooperatively with cell surface heparan sulfate on phagocytes to promote necrotic cell uptake. J Leukoc Biol 88, 559-569 (2010).
- Quah, B.J. & Parish, C.R. The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation. J Vis Exp (2010).
- Sun, R.C., et al. Reversal of the glycolytic phenotype by dichloroacetate inhibits metastatic breast cancer cell growth in vitro and in vivo. Breast Cancer Res Treat 120, 253-260 (2010).
- Smith PN, Freeman C, Yu D, Chen M, Gatenby PA, Parish CR, Li RW. 2010. Heparanase in primary human osteoblasts. J Orthop Res, 28:1315-1322.
- Yu, D, Rao, S, Tsai, LM, Lee, SK, He, YQ, Sutcliffe, EL, Srivastava, M, Linterman, M, Zheng, L, Simpson, N, Ellyard,JI, Parish, IA, Ma, CS, Li, QJ, Parish, CR, Mackay, CR, Vinuesa, CG, 2009, 'The Transcriptional Repressor Bcl-6 Directs T Follicular Helper Cell Lineage Commitment', Immunity, vol. 31, pp. 457-468.
- Juelich, T, Sutcliffe, E, Denton, A et al. 2009, 'Interplay between chromatin remodeling and epigenetic changes during lineage-specific commitment to granzyme B expression', Journal of Immunology, vol. 183, pp. 7063-7072.
- Sutcliffe, EL, Parish, IA, He, YQ et al 2009, 'Dynamic histone variant exchange accompanies gene induction in T cells', Molecular and Cellular Biology, vol. 29, no. 7, pp. 1972-1986.
- Parish, C, Clydesdale, M, Quah, B et al. 2009, 'Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation', Current Protocols in Immunology, vol. 2009, no. Supplement 84, pp. 4.9-1/13.
- Sun, R, Fadia, M, Dahlstrom, J et al 2009, 'Reversal of the glycolytic phenotype by dichloroacetate inhibits metastatic breast cancer cell growth in vitro and in vivo', Breast Cancer Research and Treatment, vol. Online first, p. 8.
- Chaudhri, G, Quah, B, Wang, Y et al. 2009, 'T cell receptor sharing by cytotoxic T lymphocytes facilitates efficient virus control', PNAS - Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 35, pp. 14984-14989.
- Poon, I, Olsson, A, Hulett, M et al 2009, 'Regulation of histidine-rich glycoprotein (HRG) function via plasmin-mediated proteolytic cleavage', Biochemical Journal, vol. 424, pp. 27-37.
- Beckman, M, Freeman, C, Parish, C et al. 2009, 'Activation of cathepsin D by glycosaminoglycans', The FEBS Journal, vol. 276, pp. 7343-7352.
- Hamzah, J, Altin, J, Herringson, T et al 2009, 'Targeted Liposomal Delivery of TLR9 Ligands Activates Spontaneous Antitumor Immunity in an Autochthonous Cancer Model', Journal of Immunology, vol. 183, no. 2, pp. 1091-1098.
- Irving-Rodgers, H, Ziolkowski, A, Parish, C et al 2008, 'Molecular composition of the peri-islet basement membrane in NOD mice: a barrier against destructive insulitis', Diabetologia, vol. 51, pp. 1680-1688.
- Quah, B, Barlow, V, McPhun, V et al. 2008, 'Bystander B cells rapidly acquire antigen receptors from activated B cells by membrane transfer', PNAS - Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 11, pp. 4259-4264.
- Sydnes, M, Orlov, A, Burns, C et al. 2008, 'Synthesis and biological evaluation of some enantiomerically pure C8c-C15-monoseco analogues of the phenanthroquinolizidine-type alkaloids cryptopleurine and julandine', Australian Journal of Chemistry, vol. 61, no. 7, pp. 506-520.
- Li RW, Freeman C, Yu D, Hindmarsh EJ, Tymms KE, Parish CR, Smith PN. 2008. Dramatic regulation of heparanase activity and angiogenesis gene expression in synovium from patients with rheumatoid arthritis. Arthritis Rheum, 58:1590-1600.
- Simson, L, Ellyard, J, Dent, L et al. 2007, 'Potential role for eosinophils in tumor immune surveillance', in Jorge Kalil, Edecio Cinha-Neto and Luiz vicente Rizzo (ed.), Proceedings 13th International Congress of Immunology, Medimond, Bologna, Italy, pp. 161-165.
- Ellyard, J, Simson, L, Orlov, A et al. 2007, 'Eotaxin Selectively Binds Heparin: An interaction that protects eotaxin from proteolysis and potentiates chemotactic activity in vivo', Journal of Biological Chemistry, vol. 282, no. 29, pp. 15238-15247.
- Quah, B, Warren, H & Parish, C 2007, 'Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester', Nature Protocols, vol. 2, no. 9, pp. 2049-2056.
- Ellyard, J, Simson, L & Parish, C 2007, 'Th2-mediated anti-tumour immunity: Friend or foe?', Tissue Antigens, vol. 70, pp. 1-11.
- Simson, L, Ellyard, J, Dent, L et al. 2007, 'Regulation of Carcinogenesis by IL-5 and CCL11: A Potential Role for Eosinophils in Tumor Immune Surveillance', Journal of Immunology, vol. 178, pp. 4222-4229.
- Parish, C 2006, 'The role of heparan sulphate in inflammation', Nature Reviews Immunology, vol. 6, no. 9, pp. 633-643.
- Adams, Y, Freeman, C, Schwartz-Albiez, R et al 2006, 'Inhibition of Plasmodium falciparum growth in vitro and adhesion to chondroitin-4-sulfate by the heparan sulfate mimetic PI-88 and other sulfated oligosaccharides', Antimicrobial Agents and Chemotherapy, vol. 50, no. 8, pp. 2850-2852.
- Altin, J & Parish, C 2006, 'Liposomal vaccines - targeting the delivery of antigen', Methods, vol. 40, pp. 39-52.
- Manderson, A, Quah, B, Botto, M et al. 2006, 'A novel mechanism for complement activation at the surface of B cells following antigen binding', Journal of Immunology, vol. 177, pp. 5155-5162.
- Banwell, M, Orlov, A, Burns, C et al 2006, 'C8c-C15 monoseco-analogues of the phenanthroquinolizidine alkaloids julandine and cryptopleurine exhibiting potent anti-angiogenic properties', Bioorganic and Medicinal Chemistry Letters, vol. 16, no. 1, pp. 181-185.
- Djordjevic, M, Parish, C, Rolfe, B et al 2006, Moulators of aniogenesis, unknown, Australia.
- Parish, C 2005, 'Heparan sulfate and inflammation', Nature Immunology, vol. 6, no. 9, pp. 861-862.
- Joyce, J, Freeman, C, Meyer-Morse, N et al 2005, 'A functional heparan sulfate mimetic implicates both heparanase and heparan sulfate in tumour angiogenesis and invasion in a mouse model of multistage cancer', Oncogene, vol. 24, pp. 4037-4051.
- Jones, A, Hulett, M & Parish, C 2005, 'Histidine-rich glycoprotein: A novel adaptor protein in plasma that modulates the immune, vascular and coagulation systems', Immunology and Cell Biology, vol. 83, no. 2, pp. 106-118.
- Freeman, C, Liu, L, Banwell, M et al 2005, 'Use of sulfated linked cyclitols as heparan sulfate mimetics to probe the heparin/heparan sulfate binding specificity of proteins', Journal of Biological Chemistry, vol. 280, no. 10, pp. 8842-8849.
- Warren, H, Jones, A, Freeman, C et al 2005, 'Evidence that the cellular ligand for the human NK cell activation receptor NKp30 is not a heparan sulfate glycosaminoglycan', Journal of Immunology, vol. 175, pp. 207-212.
- Jones, A, Poon, I, Hulett, M et al 2005, 'Histidine-rich glycoprotein specifically binds to necrotic cells via its amino-terminal domain and facilitates necrotic cell phagocytosis', Journal of Biochemistry, vol. 280, no. 42, pp. 35733-35741.
- Xie, W, McCahon, P, Jakobsen, K et al. 2004, 'Evaluation of the ability of digital infrared imaging to detect vascular changes in experimental animal tumours', International Journal of Cancer, vol. 108, no. 5, pp. 790-794.
- Nyberg, K, Ekblad, M, Bergstrom, T et al 2004, 'The low molecular weight heparan sulfate-mimetic, PI-88, inhibits cell-to-cell spread of herpes simplex', Antiviral Research, vol. 63, pp. 15-24.
- Jones, A, Hulett, M & Parish, C 2004, 'Histidine-rich glycoprotein binds to cell-surface heparan sulfate via its N-terminal domain following Zn2+ chelation', Journal of Biological Chemistry, vol. 279, no. 29, pp. 30114-30122.
- Van Broekhoven, C, Parish, C, Demangel, C et al 2004, 'Targeting dendritic cells with antigen-containing liposomes: A highly effective procedure for induction of antitumor immunity and for tumor immunotherapy', Cancer Research, vol. 64, pp. 4357-4365.
- Khachigian, L & Parish, C 2004, 'Phosphomannopentaose Sulfate (PI-88): Heparan Sulfate Mimetic with clinical potential in multiple vascular pathologies', Cardiovascular Drug Reviews, vol. 22, no. 1, pp. 1-6.
- Jones, A, Hulett, M, Altin, J et al 2004, 'Plasminogen is tethered with high affinity to the cell surface by the plasma protein, histidine-rich glycoprotein', Journal of Biological Chemistry, vol. 279, no. 37, pp. 38267-38276.
- Altin, J, Van Broekhoven, C & Parish, C 2004, 'Targeting dendritic cells with antigen-containing liposomes: antitumour immunity', Expert Opinion on Biological Therapy, vol. 4, no. 11, pp. 1735-1747.
- Levidiotis, V, Freeman, C, Punler, M et al 2005, 'A synthetic heparanase inhibitor reduces proteinuria in passive Heymann nephritis', Journal of the American Society of Nephrology, vol. 15, pp. 2882-2892.
- Francis, D, Parish, C, McGarry, M et al 2003, 'Blockade of vascular smooth muscle cell proliferation and intimal thickening after balloon injury by the sulfated oligosaccharide PI-88', Circulation Research, vol. 92, no. 8, pp. e70-e77.
- Mattes, J, Hulett, M, Xie, W et al. 2003, 'Immunotherapy of Cytotoxic T Cell-resistant Tumors by T Helper 2 Cells: An Exotaxin and STAT6-dependent Process', Journal of Experimental Medicine, vol. 197, no. 3, pp. 387-393.
- Parish, C 2003, 'Cancer immunotherapy: The past, the present and the future', Immunology and Cell Biology, vol. 81, pp. 106-113.
- Banwell, M, Orlov, A, Chand, S et al 2003, 'Convergent Synthesis and Preliminary Biological Evaluations of the Stilbenolignan (±)-Aiphanol and Various Congeners', Organic and Biomolecular Chemistry, vol. 1, no. 14, pp. 2427-2429.
- Banwell, M, Liu, L, Parish, C et al 2003, Linked cyclitols and their polysulfated derivatives, WO03004454, Australia.
- Parish, C & Altin, J 2003, In vivo targeting of dendritic cells, AU2004266034, Australia.
- Armitt, D, Banwell, M, Freeman, C et al 2002, 'C-glycoside formation via Lewis acid promoted reaction of O-glycosylimidates with pyrroles', Journal of the Chemical Society, Perkin Transactions 1, vol. n/a, no. 15, pp. 1743-1745.
- Vlodavsky, I, Friedmann, Y, Elkin, M et al 2002, 'Mammalian heparanase: molecular properties, inhibition and involvement in tumour metastasis and angiogenesis', in Foidart, J-M and Muschel, R J (ed.), Proteases and their inhibitors in cancer metastasis, Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 225-250.
- Freeman, C, Hulett, M & Parish, C 2001, 'Heparanase', in Claret, L. (ed.), Encyclopedia of Molecular Medicine, John Wiley & Sons Inc, New York, pp. 1582-1585.
- Hindmarsh, E, Staykova, M, Willenborg, D et al 2001, 'Cell surface expression of the 300 kDa mannose-6-phosphate receptor by activated T lymphocytes', Immunology and Cell Biology, vol. 79, no. 5, pp. 436-443.
- Parish, C, Pagler, E, Hornby, J et al. 2001, 'Isolation, tissue distribution, and chromosomal localization of a novel testis-specific human four-transmembrane gene related to CD20 and FceR1-b', Biochemical and Biophysical Research Communications, vol. 280, pp. 374-379.
- Manderson, A, Pickering, M, Botto, M et al. 2001, 'Continual low-level activation of the classical complement pathway', Journal of Experimental Medicine, vol. 194, no. 6, pp. 747-756.
- Parish, C & Warren, H 2001, 'Use of the intracellular fluorescent dye 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte migration and proliferation', in Coligan, J.E.; Kruisbeek, A.M. ; Margulies, D.H.; Shevach, E.M.; Strober, W. (ed.), Current Protocols in Immunology, John Wiley & Sons Inc, New York, pp. Unit 4.9, Supplement 42.
- Wall, D, Douglas, S, Ferro, V et al 2001, 'Characterisation of the anticoagulant properties of a range of structurally diverse sulfated oligosaccharides', Thrombosis Research, vol. 103, no. 4, pp. 325-335.
- Parish, C, Freeman, C & Hulett, M 2001, 'Heparanase: a key enzyme involved in cell invasion', Biochimica et Biophysica Acta: vol. 1471, no. 3, pp. M99-M108.
- Hulett, M & Parish, C 2000, 'Murine histidine-rich glycoprotein: Cloning, characterisation and cellular origin', Immunology and Cell Biology, vol. 78, no. 3, pp. 280-287.
- Hulett, M, Hornby, J, Ohms, S et al. 2000, 'Identification of active site residues of the pro-metastatic endoglycosidase heparanase', Biochemistry, vol. 39, no. 51, pp. 15659-15667.
- Van Broekhoven, C, Parish, C, Vassiliou, G et al 2000, 'Engrafting Costimulator Molecules onto Tumor Cell Surfaces with Chelator Lipids: A Potentially Convenient Approach in Cancer Vaccine Development', Journal of Immunology, vol. 164, pp. 2433-2443.
- Gorgani, N, Altin, J & Parish, C 1999, 'Histidine-rich glycoprotein regulates the binding of monomeric IgG and immune complexes by moncytes', International Immunology, vol. 11, no. 8, pp. 1275-1282.
- Gorgani, N, Altin, J & Parish, C 1999, 'Histidine-rich glycoprotein prevents the formation of insoluble immune complexes by rheumatoid factor', Immunology, vol. 98, pp. 456-463.
- Parish, C 1999, 'Fluorescent dyes for lymphocyte migration and proliferation studies', Immunology and Cell Biology, vol. 77, pp. 449-508.
- Parish, C, Freeman, C, Brown, K et al 1999, 'Identification of sulfated oligosaccharide-based inhibitors of tumour growth and metastasis using novel in vitro assays for angiogenesis and heparanase activity', Cancer Research, vol. 59, pp. 3433-3441.
- Freeman, C, Browne, A & Parish, C 1999, 'Evidence that platelet and tumour heparanases are similar, if not identical enzymes', Biochemical Journal, vol. 342, pp. 361-368.
- Smith, J, Hart, P, Parish, C et al 1999, 'Experimental melanin-induced uveitis in the Fischer 344 rats is inhibited by anti-CD4 monoclonal antibody, but not by mannose-6-phosphate', Clinical and Experimental Immunology, vol. 15, pp. 64-71.
- Gorgani, N, Parish, C & Altin, J 1999, 'Differential binding of histidine-rich glycoprotein (HRG) to human IgG subclasses and IgG molecules containing kappa and lambda light chains', Journal of Biological Chemistry, vol. 274, pp. 29633-29640.
- Baker, E, Crawford, J, Sutherland, G et al 1999, 'Human Hpa endoglycosidase heparanase Map position 4q21.3', Chromosome Research, vol. 7, p. 319.
- Hulett, M, Freeman, C, Hamdorf, B et al 1999, 'Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis', Nature Medicine, vol. 5, no. 7, pp. 803-809.
Projects and Grants
Grants information is drawn from ARIES. To add or update Projects or Grants information please contact your College Research Office.
- Understanding how heparan sulfate mimetics control neuroinflammation (Secondary Investigator)
- NHMRC 2021 Equipment Grant - Exoid (Izon Biosciences) - Linked to ARIES ID 39296 (Secondary Investigator)
- Histone Inhibition Project Extension (HIPE) "Exploiting the Novel Activities of DNA-Free and NET-associated Histones" (Primary Investigator)
- Extracellular matrix in the pathogenesis of human type 1 diabetes (Secondary Investigator)
- Targeting cancer metabolism to reduce metastases (Secondary Investigator)
- Blockade of platelet-neutrophil interactions for T1D prevention and treatment (Secondary Investigator)
- Neutrophil extracellular traps as a therapeutic target in Multiple Sclerosis (Secondary Investigator)
- The ACRF Department of Cancer Biology and Therapeutics (Secondary Investigator)
- Biomarker for beta cell destruction in Type 1 diabetes (DTF215) (Secondary Investigator)
- Extracellular matrix in the pathogenesis of human type 1 diabetes (Secondary Investigator)
- Role of the CD8-Heparan Sulfate Interaction in CD8+ T cell Development and Function (Primary Investigator)
- Novel Mechanisms, Molecular Targets and therapies in Cardiovascular Disease (Secondary Investigator)
- Beta Therapeutics Annexure 1,2,5,6,7,8 - Virtual Genomics Funding (Primary Investigator)
- In-vivo, high-resolution, whole animal imaging (Primary Investigator)
- Mechanism of protection of islet beta cells from T1D by heparan sulfate (Secondary Investigator)
- Analysis of antigen receptor sharing by T and B lymphocytes (Primary Investigator)
- New Functions for Bioactive Flavonoids in Plants and Mammals (Secondary Investigator)
- Multiphoton confocal microscope (Secondary Investigator)
- Molecular remodeling of isolated islets for optimal survival and function of islet transplants (Secondary Investigator)
- Multiphoton confocal microscope (Secondary Investigator)
- Microwave assisted peptide synthesiser (See 18298) (Primary Investigator)
- Role of heparan sulfate, heparanase and heparanase inhibitors in the development and prevention of Type I Diabetes (Primary Investigator)
- Antigen receptor sharing by lymphocytes during an immune response (Primary Investigator)
- Vascular Biology (Secondary Investigator)
- General Support for Research in JCSMR (Primary Investigator)
