Dr W M (Steve) Lee
Areas of expertise
- Biological Physics 029901
- Optical Physics 0205
- Fluidisation And Fluid Mechanics 091504
- Cell Physiology 320801
- Cellular Interactions (Incl. Adhesion, Matrix, Cell Wall) 310105
- Bioinformatic Methods Development 310201
- Artificial Intelligence And Image Processing 0801
- Biomedical Instrumentation 090303
- Medical Biotechnology Diagnostics (Incl. Biosensors) 100402
- Cardiovascular Medicine And Haematology 1102
Research interests
ANU Optical Biofluidics Group (Lee group@JCSMR)
Optical Biofluidics Imaging Group is dedicated to advancing the understanding of how cells interact and respond to fluidic forces by developing and using low phototoxic optical tools. Our work helps us to gain insight into tissue injury, pathogen invasion, and cancer metastasis under fluid mechanical forces.
A central dogma in cell migration is that chemotaxis (Brownian, osmotic) and durotaxis (material stiffness) are key extracellular drivers of cell migration. Cells live in an environment filled with viscous flowing fluid. We argue that extracellular fluid properties not only control rate of diffusion, but shapes physical forces that a cell experiences. It is therefore plausible that fluid mechanical forces precede other taxis. We focus our effort in developing new technological advances in numerical modelling, devices, imaging technologies, and biomaterials to open new ways to understand the role of fluid pressure at all levels of a living biological system.
Biography
I am an optical biophysicist who draws inspiration from the physical world of light and fluids to develop optical tools for cell biology. In my PhD (2006-2010), I engineered fluid singularities in laser fields that enhances optical tweezers (Nature Protocol- 2007) that can be used to study opto-acoustic gene transfection in mammalian cells (App Phys Lett- 2011). In parallel, I invented an ultrasensitive optical fiber-capillary scheme to test optical nonlinearity of nanofluids (Opt Express -2009, Physical Review A,-2009) and established the experimental foundation of artificial Kerr medium - first observed in 1981 by Arthur Askin (Nobel Prize Physics-2018).
In 2010-2012, I crossed discipline from optics to biomedicine at Harvard Medical School. I led the development of adaptive microendoscopy optics techniques (Massachusetts General Hospital- Advanced Microscopy Core), that is further applied to a board range of disease mice models from aortic allografts (Am J Transplant-2012), taste sensation (Sci Report -2013) and colorectal tumor development (Nat Protocol -2012).
Since 2013, I led the establishment of biomedical optics at ANU. Shortly, after my invention of a fluidic-lenses shaping kits for smartphone microscopy (2014 ANSTO Innovative Technology Award), I won successive competitive grants (2016-2023, $2.87 million as lead PI and $12.6 million as co-PI). Currently, I lead the development of Spatial Adaptive Imaging and lithography (SAIL) tools that are designed to create new cell-based assays that can accelerate the micro-Avatars (organiod, spheriods) for human disease diagnosis and therapy.
Researcher's projects
Complete List of Published Work in ORCID: https://orcid.org/0000-0002-3912-6095 , †Corresponding
A central dogma in cell migration is that chemotaxis (Brownian, osmotic) and durotaxis (material stiffness) are key extracellular drivers of cell migration. Cells live in an environment filled with viscous flowing fluid. We argue that extracellular fluid (ECF) properties not only control rate of diffusion, but shapes physical forces that a cell experiences. It is therefore plausible that fluid mechanical forces precede other taxis. To investigate ECF with single cell resolution from in vivo to in vitro, we develop and use a series of 4D optical and computational tools which we term as Spatial Adaptive Imaging (SAI) Platform.
Single Cell Fluidic Imaging We focus our effort in developing new technological advances in numerical modelling, devices, imaging technologies, and biomaterials to open new ways to understand the role of fluid pressure at all levels of a living biological system. Our goal is to develop new biological understanding of fluid-mediated mechanical forces (hydrostatic, hydrodynamic) and its prominence in promoting biological processes such as cell migration and aggregation, influencing gene expressions that reshape tissue and influence organ development, as well as informing disease progression in metastasis and thrombosis.
PUBLICATIONS
“Patterning Fluid Shear Stress Landscapes with Multiphoton Inner Laser Lithography (MILL) for Live Cell Adhesion and Translocation” Lim, Y.J., Xu, T., Zhang, J., Lin, H., , Li, Y., Zhang, Z., Hicks, S.M., Chudinov, I., Nechipurenko, D.Y., Gardiner, E.E., T Phan, W. M. Lee† bioRxiv 2022.06.17.496569
“Combined scattering, interferometry and fluorescence oblique illumination for live cell nanoscale imaging” Y Zheng*, Y J Lim*, H Lin, T Xu, C Longbottom , V Delghingaro-Augusto, Y L Thong, C Parish, E E Gardiner, W. M. Lee† ACS Photonics 9, 12, 3876–3887 (2022)
“Label-free multimodal quantitative imaging flow assay for intrathrombus formation in vitro.” Y. Zheng, S. J. Montague, Y. J. Lim, T. Xu, T. Xu, E. E. Gardiner and W. M. Lee† (2021). Biophys J 120(5): 791-804.
“Modified inverted selective plane illumination microscopy for sub-micrometer imaging resolution in polydimethylsiloxane soft lithography devices.” T. Xu, Y. J. Lim, Y. Zheng, M. Jung, K. Gaus, E. E. Gardiner and W. M. Lee† (2020)Lab Chip 20(21): 3960-3969.
“Holo-UNet: hologram-to-hologram neural network restoration for high fidelity low light quantitative phase imaging of live cells.” Z. Zhang, Y. Zheng, T. Xu, A. Upadhya, Y. J. Lim, A. Mathews, L. Xie and W. M. Lee† (2020). Biomed Opt Express 11(10): 5478-5487.
“Quantifying Embolism: Label-Free Volumetric Mapping of Thrombus Structure and Kinesis in a Microfluidic System with Optical Holography.”X. F. He, S. J. Montague, X. Tao, E. E. Gardiner and W. M. Lee† (2018). " Advanced Biosystems 2(10).
Tissue-level Fluidic imaging In vivo imaging studies of cell-fluids interactions are essential to test particular hypotheses and avoid the reductionist approach taken in in vitro systems. The next generation of deep tissue level fluid imaging need to provide sufficient visualisation for multiple cell population tracking under varying fluid stress. Within SAI platform, we have the next generation of deep tissue in vivo imaging tools which combines the latest in laser scanning methods, automation technologies, computer vision and machine learning techniques.
PUBLICATIONS
"Computational single-objective scanning light sheet (cSOLS)", T Xu, H Lin,Y J Lim, P R. Nicovich, K Gaus, and W.M.Lee†, APL Photonics 7, 081302 (2022)
"Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption."M. M. McDonald, W. H. Khoo, P. Y. Ng, … W. M. Lee, … P. I. Croucher and T. G. Phan. Cell 184(7): 1940 (2021)
“Raster Scanning Adaptive Optics for video rate correction and large field of view imaging” Y X Li, Y J Lim, Q K Xu, L Beattie, E E Gardiner, K Gaus, W Heath, W.M.Lee†, “” Biomedical Optics Express 2 1032 (2020)
“Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging." Y. X. Li, V. Gautam, A. Brustle, I. A. Cockburn, V. R. Daria, C. Gillespie, K. Gaus, C. Alt and W. M. Lee† (2017). J Biophotonics 10(11): 1526-1537.
“High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope” Y X Li, S.J. Montague, A Brüstle, X.F. He, C. Gillespie, K Gaus, E.E. Gardiner, W.M.Lee† “ Journal of Biophotonics 11 (7), e201700341 (2018)
“In vivo cellular imaging of internal organs in small animals with GRIN fluorescence endomicroscopy: from fabrication to imaging”J K Kim*, W.M Lee* et al, Nature Protocols 7 1456-1469(2012)
Tools from the laboratory have been the subject of 4 international patents (WO2015113105A1, WO2017205892A1, WO2018045409A1, AUSIP-2019904929). Aside from that, our team regularly get invited to give oral presentations at over 22 international and national conferences (plenary, keynote, public lectures) organised by Google, ANSTO, Royal Society-NSW, TedXCanberra, National Optical Societies (OSA, EOS, AOS) and Fraunhofer institute and Czech Academy of Sciences. Our work received media coverage in TIME, ABC News, Sunrise (Ch7) ,SMH, The Australian, CBS News and Smithsonian.
Available student projects
Open until filled
Seeing cells move under fluid pressure & Beating the limits of Phototoxicity
Our mission is to develop adaptive, low phototoxicity imaging tools that can map out long range directed cell migration under fluid pressure (static and dynamic) that occurs during organ development, injury and infection.Fluid-mediated mechanical forces (hydrostatic, hydrodynamic) have emerged as a prominent player in promoting biological processes such as cell migration and aggregation, influencing gene expressions that reshape tissue and influence organ development, as well as informing disease progression in metastasis and thrombosis.
To fulfil our mission, we shall be recruiting three PhD candidates join a small and highly dynamic team of researchers (biophysicists, biochemists, computational biologists) to work on the following projects
1. Single Cell-level Fluidics Imaging
Stromal cell aggregating and migrating under changing extracellular fluid pressure (ECF)
This PhD will work in an international group of biophysicists, and lead in the application of bespoken quantitative bioimaging technologies to study how hydrostatic and hydrodynamic fluid forces influence long range migratory pattern of mammalian cells. The student will study how individual and groups of stromal cells modify their cytoskeleton (through transmembrane proteins) to move into a 3D ECF rich environment. Novel 4D computational single cell approaches will be explored.
2. Tissue-level Fluidics Imaging
Tracking and study cell migrate in ECF rich microenvironment.
Cells migrating in microenvironment encounters soft fibrils matrix and changing fluid pressure under active flow and varying viscosity. This PhD will work with group of biophysicists, cancer biologists, immunologists, to develop study how blood cells, immune cells and cancer migrating within living organs (lymph node, blood vessels and tumour) using actomyosin machinery that alters local micronenvironment. Novel adaptive imaging solutions using cutting edge multiphoton tools and new computational imaging packages will be used to study these processes.
3. Engineering ECF for 3D self assembly of spheroid and organoid.
Disease Avatars in a bubble
Through our understanding of cell movement, aggregating in both in vitro and in vivo settings, alongside with our Spatial Adaptive Imaging and lithography (SAIL) tools, we will design to create new cell-based assays that can accelerate, we set out to create micro-Avatars (organiod, spheriods) to be used for human disease diagnosis and therapy. This PhD will be focus on using active flow with engineered synthetic fluids to developed template-free 3D spheroid and organoid.
Unlimited access to cutting edge biophysical tools: Both candidates will have unlimited access to a host of industry-grade 4D optical imaging and computational platforms developed and designed by the Biofluidic Group (OBIG) that are located at John Curtin School of Medical Research (JCSMR) at the Australian National University – Canberra, ACRF INCITE Centre at Garvan Institute of Medical Research – Sydney, Doherty Institute at Melbourne – ARC LIEF. Projects will be embedded in JCSMR, Garvan and Doherty graduate programs with excellent supervision and a dynamic and international community of international PhD students.
Our expectations: Applicants are required to hold a 1st Class Honours or Master’s degree in the biophysics and/or biological sciences or other basic science disciplines in physics, chemistry and biology. A good command of the English language is necessary. We also welcome cell biologists with previous experience in advanced fluorescence microscopy as well as computational biologists with experience advanced image data analysis. At the ANU, we strongly support equal opportunity and diversity. We welcome all applicants regardless of sex, nationality, ethnic or social background, religion or worldview, disability, age, sexual orientation or gender identity. We are committed to creating family-friendly working conditions. We actively encourage applications by women.
Please send an electronic application including your CV, a short motivation letter and two references to Dr W M Steve Lee
Current student projects
- Dr Daniel Lim - Senior Imaging Scientist, Adaptive Imaging and Lithography for Cell Migration
- Mr Tienan Xu- PhD student, Volumetric 4D Imaging with Computers and Light
- Mr Makoto Bannon - Medical Science Intern, Extracellular Fluid on Cell-Cell Communication and Aggregation
- Mr Kieren Dyke, Imaging Intern, Computational Imaging for Live Cell
Past student projects
Through the comprehensive research and teaching program in Optical Biofluidics Imaging Group at ANU, we have trained and graduated over 41 researchers (postdoctoral, PhDs, Masters, Honors Project, summer scholars) that has taken up senior research or industry positions - tenure track position at Beijing University of Posts and Telecommunications, postdoctoral position at Rockefeller University, University of Adelaide, University research fellowship (Birmingham) and EMBO fellowship. Positions in established biomedical industry including Therapeutic Goods Administration (TGA), Cochlear Australia , ResMed (Sydney), Beijing Genomics Institute. Research undergraduates from the laboratory have obtained highest distinction from Australian National University, Institution of Engineering and Technology and Harvard-MIT Program in Health Sciences and Technology.
Junior Research Staff (Primary Supervisor)
2020- 2023 : Mr Zhiduo Zhang ,Snr CVR, Droneshield
2022 - 2023: Mr Andrew Waddell, Royal Australian Navy
2021 - 2022: Mr Hanqi Lin , UC Santa Cruz (MSc)
2021 - 2022: Mr Kwun Ting (Jasper) Li , ANU Academic Tutor
2020 - 2022: Mr Junxiang Zhang , ANU Academic Tutor
2017 - 2020: Mr Tao Xu , Toyota Graduate Training Program
2018 - 2019: *Dr Yuanqing (Alex) Ma , EMBO Fellow, Marseille France
2017 - 2018: Dr Samantha Montague, Research Fellow,
2016 - 2017: Dr Roland Fleddermann, Research Fellow,
2014 - 2015: Mr Zijian Cen, Senior Product Analytics
Graduate students (Primary supervisor)
2020- 2023 : Mr Zhiduo Zhang MPhil Candidate , Snr CVR, Droneshield
2017 - 2022: Mr Avinash Upadhya- PhD Candidate, Postdoctoral fellow, Uni of Adelaide
2017 - 2021: Ms Yujie Zheng, PhD Candidate, Optical Engineer, BGI/MGI
2015 - 2019: Mr Yongxiao Li, PhD Candidate, Associate Professor(Research)
2014 - 2018: Miss Xuefei (Sherry) He, PhD Candidate Imaging Engineer, HiSilicon
2014 - 2018: Miss Tahseen Kamal, PhD Candidate Research Associate UNSW
2016 - 2017: *Miss Wei Zhong, Master Student (Uni Twente, Netherlands) Research Scientist, Science Sport
2013 - 2016: Mr Niko Eckerskorn, PhD Candidate (Australian National University) Analytics Engineer - CoreLogic RP Data
2015 - 2016: *Mr Wengou Zhu, Visiting PhD candidate(Sun Yat University, China) Lecturer
2014 – 2015: *Mr Redmar Vileg, Visiting Master Student (Uni Twente, Netherlands) PhD student, Leiden Uni
2013 – 2014: Mr Huang Longyao, Master by Research (Australian National University)
2013 – 2014: *Miss Sjoukje Schoustra, Visiting Master Student (Uni Twente, Netherlands) PhD student, Twente Uni
2013 – 2014 : *Miss Li Li,Visiting PhD candidate(Sun Yat University, China) Research Engineering Huawei
Undergraduate
2020 - 2021: Mr Hanqi Lin Imaging Technician, ANU
2019 - 2020: Mr Junxiang Zhang Imaging Technician, ANU
2019 - 2020: Mr Sanjeev Parbaharan
2018 : *Ms Hui Wang Undergraduate, Tsinghua University
2017- 2018: Mr Yonglin Ren
2017- 2017: Mr Oliver Johnson, University Medal | ANU-HST-MIT summer scholar
2016 - 2016 : Ms Emily Campbell Tillyard prize recipient | ANU-HST-MIT Summer scholar
2016- 2017: Mr Tienan Xu ANU-PhD student
2015 - 2016: Mr Thomas McMenamin Bioscout-Head R&D
2015 - 2016: Mr Avinash Upadhya, University Medal | ANU-HST-MIT summer scholar ANU-PhD student
2015 - 2016: Mr Yang Lu CSIRO-PhD student
2015 - 2016: Mr Tao Xu ANU-MSc student
2014 - 2015: Mr Misha Petkovic System Engineer, Seeing Machine
2015 - 2016 Miss Yi Du
2015 - 2016 Miss Yujie Zheng ANU-PhD student
2015 - 2016 Mr Lyle Halliday, University Medal, IET Medal System Engineer, S Medical
2014 - 2015 Mr Zachary Shafron, Honorable mention(undergraduate project) System Engineer, Thales
2013 –2015 Mr Avinash Upadhya, Yao Su Student Research Award ANU-PhD student
2014 –2015 Ms Rachel Watkins,YouFab Global Creative Awards, Finalist Teacher (STEM)
2014 –2015 Mr David Wright
2014 –2015 Mr Jaden Rubstein
2014 –2015 Mr Zi Cen (Kenny) Senior Product Analytics Engineer ResMed
2014 –2015 Mr Alan Harrison
2013 –2014 Miss Siti Mohd Shariaf
* visiting
Publications
- Zheng, Y, Montague PhD, S, Lim, Y et al. 2021, 'Label-free multimodal quantitative imaging flow assay for intrathrombus formation in vitro', Biophysical Journal, vol. 120, no. 5, pp. 791-804.
- McDonald, M, Khoo, W, Ng, P et al. 2021, 'Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption', Cell, vol. 184, no. 5, pp. 1330-1347.
- Upadhya, A, Zheng, Y, Li, L et al. 2021, 'Extending tracking range through structured back focal plane interferometry', SPIE Conference 11701: Complex Light and Optical Forces XV, SPIE, California, United States, p. 8.
- Xu, T, Lim, Y, Zheng, Y et al. 2020, 'Achieving sub-micrometer imaging resolution in PDMS soft lithography devices using modified inverted selective plane illumination microscopy', 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, United States, pp. 558-560.
- Zheng, Y, Montague PhD, S, Lim, Y et al. 2020, 'Coherent Optical Scattering and Interferometry (COSI) Microscopy for Morphological Imaging of Thrombus', 2020 Conference on Lasers and Electro-Optics, CLEO 2020, ed. John Wallace, Institute of Electrical and Electronics Engineers Inc., Piscataway, New Jersey, pp. 1-3.
- Li, Y, Lim, Y, Xu, Q et al. 2020, 'Raster Adaptive Optics for Video Rate Laser Scanning Microscopy with Large Field of View Correction', 2020 Conference on Lasers and Electro-Optics, CLEO 2020, ed. John Wallace, Institute of Electrical and Electronics Engineers Inc., Piscataway, New Jersey, pp. 1-2.
- Zhang, Z, Zheng, Y, Xu, T et al. 2020, 'Holo-UNet: hologram-to-hologram neural network restoration for high fidelity low light quantitative phase imaging of live cells', Biomedical Optics Express, vol. 11, no. 10, pp. 5478-5487.
- Montague PhD, S, Hicks, S, Lee, C et al. 2020, 'Fibrin exposure triggers αIIbβ3-independent platelet aggregate formation, ADAM10 activity and glycoprotein VI shedding in a charge-dependent manner', Journal of Thrombosis and Haemostasis, vol. 18, no. 6, pp. 1447-1458.
- Xu, T, Lim, Y, Zheng, Y et al. 2020, 'Modified inverted selective plane illumination microscopy for sub-micrometer imaging resolution in polydimethylsiloxane soft lithography devices', Lab on a Chip, vol. 20, no. 21, pp. 3960-3969.
- Li, Y, Lim, Y, Xu, Q et al. 2020, 'Raster adaptive optics for video rate aberration correction and large FOV multiphoton imaging', Biomedical Optics Express, vol. 11, no. 2, pp. 1032-1042.
- Montague PhD, S, Lim, Y, Lee, W et al. 2020, 'Imaging Platelet Processes and Function-Current and Emerging Approaches for Imaging in vitro and in vivo', Frontiers in Immunology, vol. 11, pp. -.
- Ayyalil, F, Montague PhD, S, Hicks, S et al. 2019, 'Platelet Function in Paroxysmal Nocturnal Haemoglobinuria', 2019 ASH Annual Meeting, American Society of Hematology, Washington D.C., United States, pp. 4884-4884.
- Lui, M, Gardiner, E, Arthur, J et al. 2019, 'Novel Stenotic Microchannels to Study Thrombus Formation in Shear Gradients: Influence of Shear Forces and Human Platelet-Related Factors', International Journal of Molecular Sciences, vol. 20, no. 12, pp. -.
- Upadhya, A, Zheng, Y, Li, L et al. 2019, 'Structured Back Focal Plane Interferometry (SBFPI)', Scientific Reports, vol. 9, no. 1, pp. -.
- Wang, Y, He, X, Bruggeman, K et al. 2019, 'Peptide Programmed Hydrogels as Safe Sanctuary Microenvironments for Cell Transplantation', Advanced Functional Materials, vol. 30, no. 9, p. 1900390.
- Zhang, Z, Lee, W, Xie, L et al. 2019, 'Noise reduction in ultra-low light digital holographic microscopy using neural networks', Biophotonics Australasia 2019, ed. E M Goldys, B C Gibson, SPIE, Australia.
- Xu, T, He, X, Zhang, Z et al. 2019, 'Software package for off-Axis digital holographic microscopy imaging processing', Biophotonics Australasia 2019, ed. E M Goldys, B C Gibson, SPIE, Australia.
- Lim, Y, Li, Y & Lee, W 2019, 'Achieving 3D FRAP using multiphoton polygon scanning microscopy', Biophotonics Australasia 2019, ed. E M Goldys, B C Gibson, SPIE, Australia.
- Daria, V & Lee, W 2018, 'Optical Nanomanipulation and Structured-Beam Optical Traps', in David Andrews Thomas Nann Robert Lipson (ed.), Comprehensive Nanoscience and Nanotechnology, Elsevier Science, London, p347
- Kamal, T, Yang, L & Lee, W 2018, 'In situ retrieval and correction of aberrations in moldless lenses using Fourier ptychography', Optics Express, vol. 26, no. 3, pp. 12pp.
- Lee, W, McMenamin, T & Li, Y 2018, 'Optical toolkits for in vivo deep tissue laser scanning microscopy: a primer', Journal of Optics, vol. 20, no. 6, pp. 1-13pp.
- He, X, Montague, SJ, Gardiner, E et al 2018, 'Quantifying Embolism: Label-Free Volumetric Mapping of Thrombus Structure and Kinesis in a Microfluidic System with Optical Holography', Advanced Biosystems, 1800089
- Li, Y, Montague, SJ, Bruestle, A et al. 2018, 'High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope', Journal of Biophotonics, 11 (7), e201700341
- Fang, Q, Curatolo, A, Wijesinghe, P et al. 2017, 'Ultrahigh-resolution optical coherence elastography through a micro-endoscope: towards in vivo imaging of cellular-scale mechanics', Biomedical Optics Express, vol. 8, no. 11, pp. 5127-5138.
- Li, Y, Gautam, V, Bruestle, A et al. 2017, 'Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging', Journal of Biophotonics, vol. 10, no. 11, pp. 1526-1537.
- He, X, Gaus, K & Lee, W 2017, 'Label-free dynamic volumetric imaging of deforming giant unilamellar vesicles under micro-flows', OSA Biophotonics Congress: The Optics in Life Sciences 2017, Optical Society of American (OSA), USA, pp. 1-3pp.
- Li, Y, Huang, H, Burgio, G et al. 2017, 'Dynamic control over field of view in polygon mirror-based laser scanning multiphoton microscope', OSA Biophotonics Congress: The Optics in Life Sciences 2017, Optical Society of American (OSA), USA, pp. 1-3pp.
- Choy, J, Sane, S, Lee, W et al. 2017, 'Improving focal photostimulation of cortical neurons with pre-derived wavefront correction', Frontiers in Cellular Neuroscience, vol. 11, no. 105, pp. 105-105.
- Fang, Q, Curatolo, A, Wijesinghe, P et al. 2017, 'Ultrahigh resolution optical coherence elastography combined with a rigid micro-endoscope', Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI, ed. J G Fujimoto, J A Izatt & V V Tuchin, SPIE, Bellingham, USA, pp. 1pp.
- Fleddermann, R, Lee, W, Huang, K et al. 2017, 'Compact flexible multi-pass rotary delay line using spinning micro-machined mirrors', Scientific Reports, vol. 7, no. 9299, pp. 1 - 9pp..
- Kamal, T, Yang, L & Lee, W 2017, 'Application of computational optics in moldless lenses', 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2017, Optical Society of American (OSA), Online, pp. 1-3pp.
- McMenamin, T & Lee, W 2017, 'A compact multi-trap optical tweezer system based on CD-ROM technologies', Optical Trapping and Optical Micromanipulation XIV, ed. K Dholakia and G C Spalding, SPIE - The International Society for Optical Engineering, Bellingham, USA, pp. 1-8.
- Kamal, T, Watkins, R, Cen, Z et al. 2017, 'Design and fabrication of a passive droplet dispenser for portable high resolution imaging system', Scientific Reports, vol. 7, pp. 41482-41482.
- Kamal, T, Watkins, R, Cen, Z et al. 2016, 'Thimble microscope system', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States.
- Li, Y & Lee, W 2016, 'PScan 1.0: flexible software framework for polygon based multiphoton microscopy', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 6pp.
- He, X, Zheng, Y & Lee, W 2016, 'DHMI: Dynamic holographic microscopy interface', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 8pp.
- Li, Y, Bruestle, A, Gautam, V et al. 2016, 'High speed multiphoton imaging', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 5pp.
- Lee, W 2016, 'High Speed Temporal Modulation with Cascaded Fibre Delay Line and Micro-structured Mirrors', Australian Conference on Optical Fibre Technology (ACOFT 2016), Optical Society of American (OSA), New York, USA.
- Kamal, T, Watkins, R, Cen, Z et al. 2016, 'Direct fabrication of silicone lenses with 3D printed parts', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 1001336 (1-6pp).
- Zhu, W, Eckerskorn, N, Upadhya, A et al. 2016, 'Dynamic axial control over optically levitating particles in air with an electrically-tunable variable-focus lens', Biomedical Optics Express, vol. 7, no. 7, pp. 2902-2911.
- He, X., ...Wang, Y. et al (2016). "Automated Fourier space region-recognition filtering for off-axis digital holographic microscopy." Biomedical Optics Express 7(8): 3111-3123.
- He, X, Maier, A & Lee, W 2016, 'Mapping the progression of malaria infected erythrocytes with holographic microscopy', Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015, IEEE, TBC, pp. 1-2.
- Kamal, T, He, X & Lee, W 2015, 'Reinventing Pocket Microscopy', Journal of the Royal Microscopical Society, vol. 37, no. 4, pp. 41-43.
- Choi, M, Lee, W & Yun, S 2015, 'Intravital microscopic interrogation of peripheral taste sensation', Scientific Reports, vol. 5, pp. -.
- Lee, W, Wright, D, Watkins, R et al. 2015, 'Integrated elastic microscope device', Proceedings of SPIE - International Society for Optical Engineering, vol. 9332.
- Lee, W 2015, 'Miniature droplet lenses for mobile microscopy', SPIE Newsroom, pp. 3pp.
- Kamal, T, He, X & Lee, W 2015, 'Reinventing Pocket Microscopy', Journal of the Royal Microscopical Society, vol. 37, no. 4, pp. 41-43.
- Lee, W, Upadhya, A, Reece, P et al. 2014, 'Fabricating Low Cost and High Performance Elastomer Lenses using Hanging Droplets', Biomedical Optics Express, vol. 5, no. 5, pp. 1626-1635.
- L. Li, W. Lee, X. Xie, W. Krolikowski, A. Rode, and J. Zhou, "Shaping self-imaging bottle beams with modified quasi-Bessel beams," Opt. Lett. 39, 2278-2281 (2014).
- Lee, W 2014, 'High Performance, low cost elastomer optics', Optical Fabrication and Testing, OFT 2014, Optics Infobase, USA.
- Lee, W 2014, 'Imaging Cells in Living Animals with Two Photon In-Vivo Abberation-Free Endomicroscopy', Australian Biomedical Engineering Conference ABEC 2014, Engineers Australia, Australia.
- Lee, W 2014, 'Stick-on microscope for smartphones', Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, ed. G L Coté, SPIE, United States of America, pp. 1-6.
- Lee, W 2014, Frugal microscopy: Inspiring tomorrow's interdisciplinary scientist, pp. 24-25.
- Lee, W 2014, Pocket Pathology, pp. 39-41.
- Lee, W & Kamal, T 2014, A Droplet Approach to Lens Making, pp. 30-31.
- Lee, W, Upadhya, A & Phan, T 2014, 'Mobile microscopy on the move', SPIE Digital Photography X, ed. N Sampat, R Tezaur, S Battiato, B A Fowler, SPIE, USA, pp. 1-7.
- Eckerskorn, N, Li, L, Kirian, R et al. 2013, 'Hollow Bessel-like beam as an optical guide for a stream of microscopic particles', Optics Express, vol. 21, no. 25, pp. 30492-30499.
- F Wang, W Toe, W.M. Lee et al “Resolving Stable Axial Trapping Points of Nanowires in an Optical Tweezers Using Photoluminescence Mapping" Nanoletters 13 1185-1191 (2013)
- Li, L, Eckerskorn, N, Kirian, R et al 2013, 'Quasi-bessel hollow beam as optical guide for micro-particles', Optical Trapping and Optical Micromanipulation X, SPIE - The International Society for Optical Engineering, San Diego, CA, V.8810, pp.88100N1-88100N11.
- Kim, J, Lee, W, Kim, P et al. 2012, 'Fabrication and operation of GRIN probes for in vivo fluorescence cellular imaging of internal organs in small animals', Nature Protocols, vol. 7, no. 8, pp. 1456-1469.
- Lee, W, Chow, T & Ng, B 2011, 'Resolving inter-particle position and optical forces along the axial direction using optical coherence gating', Bio-Optics: Design and Application, BODA 2011, The Optical Society, Monterey USA.
- Arita, Y, Torres-Mapa, M, Lee, W et al. 2011, 'Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles', Applied Physics Letters, vol. 98, no. 9, pp. 1-4.
- Lee, W & Yun, S 2011, 'Adaptive aberration correction of GRIN lenses for confocal endomicroscopy', Optics Letters, vol. 36, no. 23, pp. 4608-4610.
- Ahluwalia, B & Lee, W 2010, 'Collinear non-diffracting beams: classification and properties', Complex Light and Optical Forces IV, SPIE - The International Society for Optical Engineering, San Francisco, CA, pp. 1-11.
- Shane, J, Mazilu, M, Lee, W et al. 2010, 'Effect of pulse temporal shape on optical trapping and impulse transfer using ultrashort pulsed lasers', Optics Express, vol. 18, no. 7, pp. 7554-7568.
- Chow, T, Lee, W, Tan, K et al. 2010, 'Resolving interparticle position and optical forces along the axial direction using optical coherence gating', Applied Physics Letters, vol. 97, no. 23, pp. 231113 - 231113-3.
- Mthunzi, P, Lee, W, Riches, A et al. 2010, 'Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells', IEEE Journal on Selected Topics in Quantum Electronics, vol. 16, no. 3, pp. 608 - 618.
- Tan, K, Mazilu, M, Chow, T et al. 2009, 'In-fiber common-path optical coherence tomography using a conical-tip fiber', Optics Express, vol. 17, no. 4, pp. 2375-2384.
- El-Ganainy, R, Christodoulides, D, Wright, E et al. 2009, 'Nonlinear optical dynamics in nonideal gases of interacting colloidal nanoparticles', Physical Review A - Atomic, Molecular, and Optical Physics, vol. 80, no. 5, pp. 1-6.
- Lee, W, El-Ganainy, R, Christodoulides, D et al. 2009, 'Nonlinear optical response of colloidal suspensions', Optics Express, vol. 17, no. 12, pp. 10277-10289.
- Lee, W, Dholakia, K, Wright, E et al. 2009, 'Probing the nonlinear optical response of nanosuspensions', 2009 Conference on Lasers and Electro-Optics and 2009 Conference on Quantum Electronics and Laser Science Conference, CLEO/QELS 2009, IEEE, Baltimore, MD, pp. 1-2.
- El-Ganainy, R, Christodoulides, D, Wright, E et al. 2009, 'Optical Nonlinearity of a Colloidal "Non-Ideal Gas" of Nano-Suspensions', 2009 Conference on Lasers and Electro-Optics and 2009 Conference on Quantum Electronics and Laser Science Conference, CLEO/QELS 2009, IEEE, Baltimore, MD, pp. 1-2.
- Wright, E, Lee, W, Dholakia, K et al. 2009, 'Optical nonlinearity of liquid nanosuspensions: Kerr versus exponential model', Optical Trapping and Optical Micromanipulation VI, SPIE - The International Society for Optical Engineering, San Diego, CA, p. 9.
- Lee, W, Hernandez-Pozos, J, Vera-Robles, L et al. 2009, 'Dielectric enhanced nanoparticles for three-dimensional optical manipulation', Optical Trapping and Optical Micromanipulation VI, SPIE - The International Society for Optical Engineering, San Diego, CA, pp. 1-9.
- Dholakia, K & Lee, W 2008, 'Optical Trapping Takes Shape: The Use of Structured Light Fields', Advances in Atomic, Molecular and Optical Physics, vol. 56, pp. 261-337.
- Hernandez-Pozos, J, Lee, W, Vera-Robles, L et al. 2008, 'Controlled three-dimensional manipulation of vanadium oxide nanotubes with optical tweezers', Applied Physics Letters, vol. 93, no. 34, pp. 243107 - 243107-3.
- Wright, E, Lee, W, Giscard, P et al. 2008, 'Long distance beam propagation in colloidal suspensions: Comparison between theory and experiment', Optical Trapping and Optical Micromanipulation V, ed. Kishan Dholakia and Gabriel C. Spalding, SPIE, Bellingham, WA, USA, pp. 1-9.
- Shane, J, Mazilu, M, Lee, W et al. 2008, 'Optical trapping using ultrashort 12.9fs pulses', Optical Trapping and Optical Micromanipulation V, ed. Kishan Dholakia and Gabriel C. Spalding, SPIE, Bellingham, WA, USA.
- J. L. Hernandez-Pozos, W. M. Lee, L. I. Vera-Robles, A. Campero, and K. Dholakia, "Controlled three-dimensional manipulation of vanadium oxide nanotubes with optical tweezers," Applied Physics Letters 93, 243107 (2008)
- W. M. Lee, P. J. Reece, R. F. Marchington, N. K. Metzger, and K. Dholakia, "Construction and calibration of an optical trap on a fluorescence optical microscope," Nature Protocols 2, 3226-3238 (2007)
Projects and Grants
Grants information is drawn from ARIES. To add or update Projects or Grants information please contact your College Research Office.
- Understanding and controlling thrombus formation (Secondary Investigator)
- 5D Imaging Flow Cytometry for in vivo Quantification of Biological Fluids (Primary Investigator)
- A multiplex microscope platform to define molecular events in fluid systems (Secondary Investigator)
- New bio-optical approaches to studying the regulation behind fibrin/GPVI role in thrombus formation (Secondary Investigator)
- Polyscope: Ultra-flexible polygon scanning microscope system (Primary Investigator)
- Confocal and single molecule microscopes for Systems Microscopy (Secondary Investigator)
- Multi-modal ophthalmological instruments using ultrafast time domain OCT (Secondary Investigator)
- Regulating gene delivery with light (Primary Investigator)
- Towards the first Wearable Microscope (Primary Investigator)
- Microscope for smartphones (Primary Investigator)