Dr W M (Steve) Lee
Areas of expertise
- Biological Physics 029901
- Optical Physics 0205
- Fluidisation And Fluid Mechanics 091504
- Cell Physiology 320801
- Cellular Interactions (Incl. Adhesion, Matrix, Cell Wall) 310105
- Bioinformatic Methods Development 310201
- Artificial Intelligence And Image Processing 0801
- Biomedical Instrumentation 090303
- Medical Biotechnology Diagnostics (Incl. Biosensors) 100402
- Cardiovascular Medicine And Haematology 1102
Research interests
ANU Optical Biofluidics Group (Lee group@JCSMR)
Living cells are an integrated network of genes, proteins and dynamic metabolic reactions that give rise to functions that are essential for life. Cells moves and organizes into complex networks under a diverse range of microscopic fluidic forces (interstitial and vascular flow) in response to injury, pathogen invasion and cancer.
Our mission is to develop adaptive, low phototoxicity imaging tools that can map out long range directed cell migration under fluid pressure (static and dynamic) that occurs during organ development, injury and infection.
Biography
Steve's broad research interest is focused on the application of imaging science in the field of biomedical science.
Steve earned degrees in electronics engineering (Nanyang Tech Uni), Physics (Uni St Andrews) and trained in advanced bioimaging (Harvard) and industry instrumentation (Nikon/Einst). In his undergraduate and research assistantship, he studied phase singularities in shaped light field (aka fluidic vortex of light) by pioneering optical holography and so-gel electron lithography methods. He then headed R&D department at Einst Technology Pte Ltd to develop confocal laser scanning technologies. His PhD (2010)- University of St Andrews and University of Arizona led to the invention of an optical fiber-capillary fluidics diagnostic tool that resolved the fundamental optical properties of nanofluids. In parallel, he developed fluid shear optical gene transfection in mammalian cells with laser trapping/cavitation. From 2010-2012, at Harvard and Massachusetts General Hospital (Wellman Photomedicine), he worked with Andy Yun as a NIH/NIDDK postdoctoral fellow to pioneer in vivo imaging tools that investigated micro-blood vessel flow dynamics in tumorigenesis, organ transplantation and taste sensation in living mice.
At start of his independent lab in mid-2013, he invented fluidic-lens gravity shaping principles for low cost high performance smartphone microscopy/dermatoscope and was awarded the 2014 ANSTO Innovative Technology Award by Australian Museum.
Since 2012, he has won successive competitive research fellowships and project grants (e.g.UNSW VC Fellow, ANU NIG-FERL, ARC DECRA, 2x ARC DP, 1x NHMRC Ideas, 1x ACRF, 2x CSIRO-AusIndustry, 1x PBCRC-Biosecurity).
Researcher's projects
oBIG, Google Scholar , ORCID, ResearchGate
Seeing cells move under fluid pressure & Beating the limits of Phototoxicity
Our mission is to develop adaptive, low phototoxicity imaging tools that can map out long range directed cell migration under fluid pressure (static and dynamic) that occurs during organ development, injury and infection.
Available student projects
To fulfil our mission, we shall be recruiting two PhD candidates join a small and highly dynamic team of researchers (biophysicists, biochemists, computational biologists) to work on the following projects
1. Mapping long range cell migration and memory under changing extracellular fluid pressure
Fluid-mediated mechanical forces (hydrostatic, hydrodynamic) have emerged as a prominent player in promoting biological processes such as cell migration and aggregation, influencing gene expressions that reshape tissue and influence organ development, as well as informing disease progression in metastasis and thrombosis.
This PhD will work in an international group of biophysicists, and lead in the application of bespoken quantitative bioimaging technologies to study how hydrostatic and hydrodynamic fluid forces influence long range migratory pattern of mammalian cells. Novel 4D computational single cell approaches will be explored.
2. Adaptive low phototoxicity Imaging of migrating cells in small animals
Phototoxicity describes the phenomenon that light induces undesirable physiological changes in the observed living sample, be that single cells from a cell culture or a multicellular organism. With the excitation light intensities widely used in live animal imaging, it is critical to create new optical tools that will remove significant alterations in the biochemistry, physiology and dynamic behaviour of the observed sample from light.
This PhD will work with group of biophysicists, cancer biologists, immunologists, to develop novel adaptive imaging solutions using cutting edge multiphoton tools and new computational imaging packages to study processes of cell migrating in living organs.
Unlimited access to cutting edge biophysical tools: Both candidates will have unlimited access to a host of industry-grade 4D optical imaging and computational platforms developed and designed by the Biofluidic Group (OBIG) that are located at John Curtin School of Medical Research (JCSMR) at the Australian National University – Canberra, ACRF INCITE Centre at Garvan Institute of Medical Research – Sydney, Doherty Institute at Melbourne – ARC LIEF. Projects will be embedded in JCSMR, Garvan and Doherty graduate programs with excellent supervision and a dynamic and international community of international PhD students.
Our expectations: Applicants are required to hold a 1st Class Honours or Master’s degree in the biophysics and/or biological sciences or other basic science disciplines in physics, chemistry and biology. A good command of the English language is necessary. We also welcome cell biologists with previous experience in advanced fluorescence microscopy as well as computational biologists with experience advanced image data analysis. At the ANU, we strongly support equal opportunity and diversity. We welcome all applicants regardless of sex, nationality, ethnic or social background, religion or worldview, disability, age, sexual orientation or gender identity. We are committed to creating family-friendly working conditions. We actively encourage applications by women.
Please send an electronic application including your CV, a short motivation letter and two references to Dr W M Steve Lee by 1st March 2023.
Current student projects
- Dr Daniel Lim - Senior Imaging Scientist, Adaptive Optics lithography to study fluid shear driven mechanosensation
- Mr Tienan Xu- PhD student, Quantifying Biofluid cell mechanics with light, Collaborator
- Mr Zhiduo Zhang- Imaging Scientist, Machine learning for Microscopy, Collaborator – Xie, CS , Eduardo JCSMR
- Mr Andrew Waddell, ANU Imaging Technician, Raster Adaptive Optics
- Ms Felicity Lin, ANU Imaging Intern
Past student projects
Junior Research Staff (Primary Supervisor)
2021 - 2022: Mr Hanqi Lin , UC Santa Cruz (MSc)
2021 - 2022: Mr Kwun Ting (Jasper) Li , ANU Academic Tutor
2020 - 2022: Mr Junxiang Zhang , ANU Academic Tutor
2017 - 2020: Mr Tao Xu , Toyota Graduate Training Program
2018 - 2019: *Dr Yuanqing (Alex) Ma , EMBO Fellow, Marseille France
2017 - 2018: Dr Samantha Montague, Research Fellow,
2016 - 2017: Dr Roland Fleddermann, Research Fellow,
2014 - 2015: Mr Zijian Cen, Senior Product Analytics
Graduate students (Primary supervisor)
2017 - 2022: Mr Avinash Upadhya- PhD student, Postdoctoral fellow, Uni of Adelaide
2017 - 2021: Ms Yujie Zheng, PhD Candidate, Optical Engineer, BGI/MGI
2015 - 2019: Mr Yongxiao Li, PhD Candidate, Associate Professor(Research)
2014 - 2018: Miss Xuefei (Sherry) He, PhD Candidate Imaging Engineer, HiSilicon
2014 - 2018: Miss Tahseen Kamal, PhD Candidate Research Associate UNSW
2016 - 2017: *Miss Wei Zhong, Master Student (Uni Twente, Netherlands) Research Scientist, Science Sport
2013 - 2016: Mr Niko Eckerskorn, PhD Candidate (Australian National University) Analytics Engineer - CoreLogic RP Data
2015 - 2016: *Mr Wengou Zhu, Visiting PhD candidate(Sun Yat University, China) Lecturer
2014 – 2015: *Mr Redmar Vileg, Visiting Master Student (Uni Twente, Netherlands) PhD student, Leiden Uni
2013 – 2014: Mr Huang Longyao, Master by Research (Australian National University)
2013 – 2014: *Miss Sjoukje Schoustra, Visiting Master Student (Uni Twente, Netherlands) PhD student, Twente Uni
2013 – 2014 : *Miss Li Li,Visiting PhD candidate(Sun Yat University, China) Research Engineering Huawei
Undergraduate
2020 - 2021: Mr Hanqi Lin Imaging Technician, ANU
2019 - 2020: Mr Junxiang Zhang Imaging Technician, ANU
2019 - 2020: Mr Sanjeev Parbaharan
2018 : *Ms Hui Wang Undergraduate, Tsinghua University
2017- 2018: Mr Yonglin Ren
2017- 2017: Mr Oliver Johnson, University Medal | ANU-HST-MIT summer scholar
2016 - 2016 : Ms Emily Campbell Tillyard prize recipient | ANU-HST-MIT Summer scholar
2016- 2017: Mr Tienan Xu ANU-PhD student
2015 - 2016: Mr Thomas McMenamin Bioscout-Head R&D
2015 - 2016: Mr Avinash Upadhya, University Medal | ANU-HST-MIT summer scholar ANU-PhD student
2015 - 2016: Mr Yang Lu CSIRO-PhD student
2015 - 2016: Mr Tao Xu ANU-MSc student
2014 - 2015: Mr Misha Petkovic System Engineer, Seeing Machine
2015 - 2016 Miss Yi Du
2015 - 2016 Miss Yujie Zheng ANU-PhD student
2015 - 2016 Mr Lyle Halliday, University Medal, IET Medal System Engineer, S Medical
2014 - 2015 Mr Zachary Shafron, Honorable mention(undergraduate project) System Engineer, Thales
2013 –2015 Mr Avinash Upadhya, Yao Su Student Research Award ANU-PhD student
2014 –2015 Ms Rachel Watkins,YouFab Global Creative Awards, Finalist Teacher (STEM)
2014 –2015 Mr David Wright
2014 –2015 Mr Jaden Rubstein
2014 –2015 Mr Zi Cen (Kenny) Senior Product Analytics Engineer ResMed
2014 –2015 Mr Alan Harrison
2013 –2014 Miss Siti Mohd Shariaf
* visiting
Publications
- Zheng, Y, Montague PhD, S, Lim, Y et al. 2021, 'Label-free multimodal quantitative imaging flow assay for intrathrombus formation in vitro', Biophysical Journal, vol. 120, no. 5, pp. 791-804.
- McDonald, M, Khoo, W, Ng, P et al. 2021, 'Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption', Cell, vol. 184, no. 5, pp. 1330-1347.
- Upadhya, A, Zheng, Y, Li, L et al. 2021, 'Extending tracking range through structured back focal plane interferometry', SPIE Conference 11701: Complex Light and Optical Forces XV, SPIE, California, United States, p. 8.
- Xu, T, Lim, Y, Zheng, Y et al. 2020, 'Achieving sub-micrometer imaging resolution in PDMS soft lithography devices using modified inverted selective plane illumination microscopy', 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, United States, pp. 558-560.
- Zheng, Y, Montague PhD, S, Lim, Y et al. 2020, 'Coherent Optical Scattering and Interferometry (COSI) Microscopy for Morphological Imaging of Thrombus', 2020 Conference on Lasers and Electro-Optics, CLEO 2020, ed. John Wallace, Institute of Electrical and Electronics Engineers Inc., Piscataway, New Jersey, pp. 1-3.
- Li, Y, Lim, Y, Xu, Q et al. 2020, 'Raster Adaptive Optics for Video Rate Laser Scanning Microscopy with Large Field of View Correction', 2020 Conference on Lasers and Electro-Optics, CLEO 2020, ed. John Wallace, Institute of Electrical and Electronics Engineers Inc., Piscataway, New Jersey, pp. 1-2.
- Zhang, Z, Zheng, Y, Xu, T et al. 2020, 'Holo-UNet: hologram-to-hologram neural network restoration for high fidelity low light quantitative phase imaging of live cells', Biomedical Optics Express, vol. 11, no. 10, pp. 5478-5487.
- Montague PhD, S, Hicks, S, Lee, C et al. 2020, 'Fibrin exposure triggers αIIbβ3-independent platelet aggregate formation, ADAM10 activity and glycoprotein VI shedding in a charge-dependent manner', Journal of Thrombosis and Haemostasis, vol. 18, no. 6, pp. 1447-1458.
- Xu, T, Lim, Y, Zheng, Y et al. 2020, 'Modified inverted selective plane illumination microscopy for sub-micrometer imaging resolution in polydimethylsiloxane soft lithography devices', Lab on a Chip, vol. 20, no. 21, pp. 3960-3969.
- Li, Y, Lim, Y, Xu, Q et al. 2020, 'Raster adaptive optics for video rate aberration correction and large FOV multiphoton imaging', Biomedical Optics Express, vol. 11, no. 2, pp. 1032-1042.
- Montague PhD, S, Lim, Y, Lee, W et al. 2020, 'Imaging Platelet Processes and Function-Current and Emerging Approaches for Imaging in vitro and in vivo', Frontiers in Immunology, vol. 11, pp. -.
- Ayyalil, F, Montague PhD, S, Hicks, S et al. 2019, 'Platelet Function in Paroxysmal Nocturnal Haemoglobinuria', 2019 ASH Annual Meeting, American Society of Hematology, Washington D.C., United States, pp. 4884-4884.
- Lui, M, Gardiner, E, Arthur, J et al. 2019, 'Novel Stenotic Microchannels to Study Thrombus Formation in Shear Gradients: Influence of Shear Forces and Human Platelet-Related Factors', International Journal of Molecular Sciences, vol. 20, no. 12, pp. -.
- Upadhya, A, Zheng, Y, Li, L et al. 2019, 'Structured Back Focal Plane Interferometry (SBFPI)', Scientific Reports, vol. 9, no. 1, pp. -.
- Wang, Y, He, X, Bruggeman, K et al. 2019, 'Peptide Programmed Hydrogels as Safe Sanctuary Microenvironments for Cell Transplantation', Advanced Functional Materials, vol. 30, no. 9, p. 1900390.
- Zhang, Z, Lee, W, Xie, L et al. 2019, 'Noise reduction in ultra-low light digital holographic microscopy using neural networks', Biophotonics Australasia 2019, ed. E M Goldys, B C Gibson, SPIE, Australia.
- Xu, T, He, X, Zhang, Z et al. 2019, 'Software package for off-Axis digital holographic microscopy imaging processing', Biophotonics Australasia 2019, ed. E M Goldys, B C Gibson, SPIE, Australia.
- Lim, Y, Li, Y & Lee, W 2019, 'Achieving 3D FRAP using multiphoton polygon scanning microscopy', Biophotonics Australasia 2019, ed. E M Goldys, B C Gibson, SPIE, Australia.
- Daria, V & Lee, W 2018, 'Optical Nanomanipulation and Structured-Beam Optical Traps', in David Andrews Thomas Nann Robert Lipson (ed.), Comprehensive Nanoscience and Nanotechnology, Elsevier Science, London, p347
- Kamal, T, Yang, L & Lee, W 2018, 'In situ retrieval and correction of aberrations in moldless lenses using Fourier ptychography', Optics Express, vol. 26, no. 3, pp. 12pp.
- Lee, W, McMenamin, T & Li, Y 2018, 'Optical toolkits for in vivo deep tissue laser scanning microscopy: a primer', Journal of Optics, vol. 20, no. 6, pp. 1-13pp.
- He, X, Montague, SJ, Gardiner, E et al 2018, 'Quantifying Embolism: Label-Free Volumetric Mapping of Thrombus Structure and Kinesis in a Microfluidic System with Optical Holography', Advanced Biosystems, 1800089
- Li, Y, Montague, SJ, Bruestle, A et al. 2018, 'High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope', Journal of Biophotonics, 11 (7), e201700341
- Fang, Q, Curatolo, A, Wijesinghe, P et al. 2017, 'Ultrahigh-resolution optical coherence elastography through a micro-endoscope: towards in vivo imaging of cellular-scale mechanics', Biomedical Optics Express, vol. 8, no. 11, pp. 5127-5138.
- Li, Y, Gautam, V, Bruestle, A et al. 2017, 'Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging', Journal of Biophotonics, vol. 10, no. 11, pp. 1526-1537.
- He, X, Gaus, K & Lee, W 2017, 'Label-free dynamic volumetric imaging of deforming giant unilamellar vesicles under micro-flows', OSA Biophotonics Congress: The Optics in Life Sciences 2017, Optical Society of American (OSA), USA, pp. 1-3pp.
- Li, Y, Huang, H, Burgio, G et al. 2017, 'Dynamic control over field of view in polygon mirror-based laser scanning multiphoton microscope', OSA Biophotonics Congress: The Optics in Life Sciences 2017, Optical Society of American (OSA), USA, pp. 1-3pp.
- Choy, J, Sane, S, Lee, W et al. 2017, 'Improving focal photostimulation of cortical neurons with pre-derived wavefront correction', Frontiers in Cellular Neuroscience, vol. 11, no. 105, pp. 105-105.
- Fang, Q, Curatolo, A, Wijesinghe, P et al. 2017, 'Ultrahigh resolution optical coherence elastography combined with a rigid micro-endoscope', Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI, ed. J G Fujimoto, J A Izatt & V V Tuchin, SPIE, Bellingham, USA, pp. 1pp.
- Fleddermann, R, Lee, W, Huang, K et al. 2017, 'Compact flexible multi-pass rotary delay line using spinning micro-machined mirrors', Scientific Reports, vol. 7, no. 9299, pp. 1 - 9pp..
- Kamal, T, Yang, L & Lee, W 2017, 'Application of computational optics in moldless lenses', 3D Image Acquisition and Display: Technology, Perception and Applications, 3D 2017, Optical Society of American (OSA), Online, pp. 1-3pp.
- McMenamin, T & Lee, W 2017, 'A compact multi-trap optical tweezer system based on CD-ROM technologies', Optical Trapping and Optical Micromanipulation XIV, ed. K Dholakia and G C Spalding, SPIE - The International Society for Optical Engineering, Bellingham, USA, pp. 1-8.
- Kamal, T, Watkins, R, Cen, Z et al. 2017, 'Design and fabrication of a passive droplet dispenser for portable high resolution imaging system', Scientific Reports, vol. 7, pp. 41482-41482.
- Kamal, T, Watkins, R, Cen, Z et al. 2016, 'Thimble microscope system', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States.
- Li, Y & Lee, W 2016, 'PScan 1.0: flexible software framework for polygon based multiphoton microscopy', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 6pp.
- He, X, Zheng, Y & Lee, W 2016, 'DHMI: Dynamic holographic microscopy interface', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 8pp.
- Li, Y, Bruestle, A, Gautam, V et al. 2016, 'High speed multiphoton imaging', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 5pp.
- Lee, W 2016, 'High Speed Temporal Modulation with Cascaded Fibre Delay Line and Micro-structured Mirrors', Australian Conference on Optical Fibre Technology (ACOFT 2016), Optical Society of American (OSA), New York, USA.
- Kamal, T, Watkins, R, Cen, Z et al. 2016, 'Direct fabrication of silicone lenses with 3D printed parts', SPIE Biophotonics Australasia 2016, ed. M R Hutchinson & E M Goldys, SPIE - The International Society for Optical Engineering, Bellingham, United States, pp. 1001336 (1-6pp).
- Zhu, W, Eckerskorn, N, Upadhya, A et al. 2016, 'Dynamic axial control over optically levitating particles in air with an electrically-tunable variable-focus lens', Biomedical Optics Express, vol. 7, no. 7, pp. 2902-2911.
- He, X., ...Wang, Y. et al (2016). "Automated Fourier space region-recognition filtering for off-axis digital holographic microscopy." Biomedical Optics Express 7(8): 3111-3123.
- He, X, Maier, A & Lee, W 2016, 'Mapping the progression of malaria infected erythrocytes with holographic microscopy', Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015, IEEE, TBC, pp. 1-2.
- Kamal, T, He, X & Lee, W 2015, 'Reinventing Pocket Microscopy', Journal of the Royal Microscopical Society, vol. 37, no. 4, pp. 41-43.
- Choi, M, Lee, W & Yun, S 2015, 'Intravital microscopic interrogation of peripheral taste sensation', Scientific Reports, vol. 5, pp. -.
- Lee, W, Wright, D, Watkins, R et al. 2015, 'Integrated elastic microscope device', Proceedings of SPIE - International Society for Optical Engineering, vol. 9332.
- Lee, W 2015, 'Miniature droplet lenses for mobile microscopy', SPIE Newsroom, pp. 3pp.
- Kamal, T, He, X & Lee, W 2015, 'Reinventing Pocket Microscopy', Journal of the Royal Microscopical Society, vol. 37, no. 4, pp. 41-43.
- Lee, W, Upadhya, A, Reece, P et al. 2014, 'Fabricating Low Cost and High Performance Elastomer Lenses using Hanging Droplets', Biomedical Optics Express, vol. 5, no. 5, pp. 1626-1635.
- L. Li, W. Lee, X. Xie, W. Krolikowski, A. Rode, and J. Zhou, "Shaping self-imaging bottle beams with modified quasi-Bessel beams," Opt. Lett. 39, 2278-2281 (2014).
- Lee, W 2014, 'High Performance, low cost elastomer optics', Optical Fabrication and Testing, OFT 2014, Optics Infobase, USA.
- Lee, W 2014, 'Imaging Cells in Living Animals with Two Photon In-Vivo Abberation-Free Endomicroscopy', Australian Biomedical Engineering Conference ABEC 2014, Engineers Australia, Australia.
- Lee, W 2014, 'Stick-on microscope for smartphones', Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, ed. G L Coté, SPIE, United States of America, pp. 1-6.
- Lee, W 2014, Frugal microscopy: Inspiring tomorrow's interdisciplinary scientist, pp. 24-25.
- Lee, W 2014, Pocket Pathology, pp. 39-41.
- Lee, W & Kamal, T 2014, A Droplet Approach to Lens Making, pp. 30-31.
- Lee, W, Upadhya, A & Phan, T 2014, 'Mobile microscopy on the move', SPIE Digital Photography X, ed. N Sampat, R Tezaur, S Battiato, B A Fowler, SPIE, USA, pp. 1-7.
- Eckerskorn, N, Li, L, Kirian, R et al. 2013, 'Hollow Bessel-like beam as an optical guide for a stream of microscopic particles', Optics Express, vol. 21, no. 25, pp. 30492-30499.
- F Wang, W Toe, W.M. Lee et al “Resolving Stable Axial Trapping Points of Nanowires in an Optical Tweezers Using Photoluminescence Mapping" Nanoletters 13 1185-1191 (2013)
- Li, L, Eckerskorn, N, Kirian, R et al 2013, 'Quasi-bessel hollow beam as optical guide for micro-particles', Optical Trapping and Optical Micromanipulation X, SPIE - The International Society for Optical Engineering, San Diego, CA, V.8810, pp.88100N1-88100N11.
- Kim, J, Lee, W, Kim, P et al. 2012, 'Fabrication and operation of GRIN probes for in vivo fluorescence cellular imaging of internal organs in small animals', Nature Protocols, vol. 7, no. 8, pp. 1456-1469.
- Lee, W, Chow, T & Ng, B 2011, 'Resolving inter-particle position and optical forces along the axial direction using optical coherence gating', Bio-Optics: Design and Application, BODA 2011, The Optical Society, Monterey USA.
- Arita, Y, Torres-Mapa, M, Lee, W et al. 2011, 'Spatially optimized gene transfection by laser-induced breakdown of optically trapped nanoparticles', Applied Physics Letters, vol. 98, no. 9, pp. 1-4.
- Lee, W & Yun, S 2011, 'Adaptive aberration correction of GRIN lenses for confocal endomicroscopy', Optics Letters, vol. 36, no. 23, pp. 4608-4610.
- Ahluwalia, B & Lee, W 2010, 'Collinear non-diffracting beams: classification and properties', Complex Light and Optical Forces IV, SPIE - The International Society for Optical Engineering, San Francisco, CA, pp. 1-11.
- Shane, J, Mazilu, M, Lee, W et al. 2010, 'Effect of pulse temporal shape on optical trapping and impulse transfer using ultrashort pulsed lasers', Optics Express, vol. 18, no. 7, pp. 7554-7568.
- Chow, T, Lee, W, Tan, K et al. 2010, 'Resolving interparticle position and optical forces along the axial direction using optical coherence gating', Applied Physics Letters, vol. 97, no. 23, pp. 231113 - 231113-3.
- Mthunzi, P, Lee, W, Riches, A et al. 2010, 'Intracellular Dielectric Tagging for Improved Optical Manipulation of Mammalian Cells', IEEE Journal on Selected Topics in Quantum Electronics, vol. 16, no. 3, pp. 608 - 618.
- Tan, K, Mazilu, M, Chow, T et al. 2009, 'In-fiber common-path optical coherence tomography using a conical-tip fiber', Optics Express, vol. 17, no. 4, pp. 2375-2384.
- El-Ganainy, R, Christodoulides, D, Wright, E et al. 2009, 'Nonlinear optical dynamics in nonideal gases of interacting colloidal nanoparticles', Physical Review A - Atomic, Molecular, and Optical Physics, vol. 80, no. 5, pp. 1-6.
- Lee, W, El-Ganainy, R, Christodoulides, D et al. 2009, 'Nonlinear optical response of colloidal suspensions', Optics Express, vol. 17, no. 12, pp. 10277-10289.
- Lee, W, Dholakia, K, Wright, E et al. 2009, 'Probing the nonlinear optical response of nanosuspensions', 2009 Conference on Lasers and Electro-Optics and 2009 Conference on Quantum Electronics and Laser Science Conference, CLEO/QELS 2009, IEEE, Baltimore, MD, pp. 1-2.
- El-Ganainy, R, Christodoulides, D, Wright, E et al. 2009, 'Optical Nonlinearity of a Colloidal "Non-Ideal Gas" of Nano-Suspensions', 2009 Conference on Lasers and Electro-Optics and 2009 Conference on Quantum Electronics and Laser Science Conference, CLEO/QELS 2009, IEEE, Baltimore, MD, pp. 1-2.
- Wright, E, Lee, W, Dholakia, K et al. 2009, 'Optical nonlinearity of liquid nanosuspensions: Kerr versus exponential model', Optical Trapping and Optical Micromanipulation VI, SPIE - The International Society for Optical Engineering, San Diego, CA, p. 9.
- Lee, W, Hernandez-Pozos, J, Vera-Robles, L et al. 2009, 'Dielectric enhanced nanoparticles for three-dimensional optical manipulation', Optical Trapping and Optical Micromanipulation VI, SPIE - The International Society for Optical Engineering, San Diego, CA, pp. 1-9.
- Dholakia, K & Lee, W 2008, 'Optical Trapping Takes Shape: The Use of Structured Light Fields', Advances in Atomic, Molecular and Optical Physics, vol. 56, pp. 261-337.
- Hernandez-Pozos, J, Lee, W, Vera-Robles, L et al. 2008, 'Controlled three-dimensional manipulation of vanadium oxide nanotubes with optical tweezers', Applied Physics Letters, vol. 93, no. 34, pp. 243107 - 243107-3.
- Wright, E, Lee, W, Giscard, P et al. 2008, 'Long distance beam propagation in colloidal suspensions: Comparison between theory and experiment', Optical Trapping and Optical Micromanipulation V, ed. Kishan Dholakia and Gabriel C. Spalding, SPIE, Bellingham, WA, USA, pp. 1-9.
- Shane, J, Mazilu, M, Lee, W et al. 2008, 'Optical trapping using ultrashort 12.9fs pulses', Optical Trapping and Optical Micromanipulation V, ed. Kishan Dholakia and Gabriel C. Spalding, SPIE, Bellingham, WA, USA.
- J. L. Hernandez-Pozos, W. M. Lee, L. I. Vera-Robles, A. Campero, and K. Dholakia, "Controlled three-dimensional manipulation of vanadium oxide nanotubes with optical tweezers," Applied Physics Letters 93, 243107 (2008)
- W. M. Lee, P. J. Reece, R. F. Marchington, N. K. Metzger, and K. Dholakia, "Construction and calibration of an optical trap on a fluorescence optical microscope," Nature Protocols 2, 3226-3238 (2007)
Projects and Grants
Grants information is drawn from ARIES. To add or update Projects or Grants information please contact your College Research Office.
- Understanding and controlling thrombus formation (Secondary Investigator)
- 5D Imaging Flow Cytometry for in vivo Quantification of Biological Fluids (Primary Investigator)
- A multiplex microscope platform to define molecular events in fluid systems (Secondary Investigator)
- New bio-optical approaches to studying the regulation behind fibrin/GPVI role in thrombus formation (Secondary Investigator)
- Polyscope: Ultra-flexible polygon scanning microscope system (Primary Investigator)
- Confocal and single molecule microscopes for Systems Microscopy (Secondary Investigator)
- Multi-modal ophthalmological instruments using ultrafast time domain OCT (Secondary Investigator)
- Regulating gene delivery with light (Primary Investigator)
- Towards the first Wearable Microscope (Primary Investigator)
- Microscope for smartphones (Primary Investigator)
