Areas of expertise

• Cellular Interactions (Incl. Adhesion, Matrix, Cell Wall) 060106
• Mycology 060505
• Protein Trafficking 060108
• Gene Expression (Incl. Microarray And Other Genome Wide Approaches) 060405
• Infectious Agents 060502
• Crop And Pasture Biochemistry And Physiology 070303
• Plant Cell And Molecular Biology 060702
• Structural Biology (Incl. Macromolecular Modelling) 060112
• Plant Pathology 060704
• Biochemistry And Cell Biology 0601

Publications

• Hee, W, Blackman, L & Hardham, A 2019, 'Characterisation of Stramenopile-specific mastigoneme proteins in Phytophthora parasitica', Protoplasma, vol. 256, no. 2, pp. 521-535.
• Zhang, X, Farah, N, Rolston, L et al. 2018, 'Crystal structure of the Melampsora lini effector AvrP reveals insights into a possible nuclear function and recognition by the flax disease resistance protein P', Molecular Plant Pathology, vol. 19, no. 5, pp. 1196-1209.
• Hardham, A & Blackman, L 2018, 'Phytophthora cinnamomi', Molecular Plant Pathology, vol. 19, no. 2, pp. 260-285.
• Branco, R, Pearsall, E, Rundle, C et al 2017, 'Quantifying the plant actin cytoskeleton response to applied pressure using nanoindentation', Protoplasma, vol. 254, no. 2, pp. 1127-1137.
• Anderson, C, Khan, M, Catanzariti, A et al 2016, 'Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini', BMC Genomics, vol. 17, no. 667, pp. 1-20.
• Blackman, L, Cullerne, D, Torre�a, P et al. 2015, 'RNA-Seq Analysis of the Expression of Genes Encoding Cell Wall Degrading Enzymes during Infection of Lupin (Lupinus angustifolius) by Phytophthora parasitica', PLOS ONE (Public Library of Science), vol. 10, no. 9, pp. e0136899-e0136899.
• Blackman, L, Cullerne, D & Hardham, A 2014, 'Bioinformatic characterisation of genes encoding cell wall degrading enzymes in the Phytophthora parasitica genome', BMC Genomics, vol. 15, no. 1, pp. 1-24.
• Hardham, A 2014, 'Functioning Cells', in B Knox, P Ladiges, B Evans & R Saint (ed.), Biology: An Australian Focus, McGraw-Hill Education (Australia) Pty Ltd, Australia, pp. 82-109pp.
• Ve, T, Williams, S, Catanzariti, A et al 2013, 'Structures of the flax-rust effector AvrM reveal insights into the molecular basis of plant-cell entry and effector-triggered immunity', PNAS - Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 43, pp. 17594-17599.
• Hardham, A 2013, 'Microtubules and biotic interactions', The Plant Journal, vol. 75, no. 2, pp. 278-289.
• Ve, T, Williams, S, Catanzariti, A et al 2013, 'Structures of the flax-rust effector AvrM reveal insights into the molecular basis of plant-cell entry and effector-triggered immunity', PNAS - Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 43, pp. 17594-17599.
• Ludowici, V, Zhang, W, Blackman, L et al 2013, 'Phytophthora Nicotianae', in Lamour, K (ed.), Phytophthora: a global perspective., CABI International, United Kingdom, pp. 113- 123.
• Hee, W, Torre�a, P, Blackman, L et al. 2013, 'Phytophthora Cinnamomi in Australia', in Lamour, K (ed.), Phytophthora: a global perspective., CABI International, United Kingdom, pp. 124- 134.
• Zhang, W, Blackman, L & Hardham, A 2013, 'Transient fusion and selective secretion of vesicle proteins in Phytophthora nicotianae zoospores', PeerJ, vol. 1, no. 1, pp. 1-24.
• Takemoto, D, Rafiqi, M, Hurley, U et al 2012, 'N-terminal motifs in some plant disease resistance proteins function in membrane attachment and contribute to disease resistance', Molecular Plant-Microbe Interactions (MPMI), vol. 25, no. 3, pp. 379-392.
• Gan, P, Dodds, P & Hardham, A 2012, 'Plant infection by biotrophic fungal and oomycete pathogens', in Perotto, Silvia; Baluska, Frantisek (ed.), Signaling and Communication in Plant Symbiosis, Springer, Berlin, pp. 183-212.
• Hardham, A 2012, 'Confocal microscopy in plant-pathogen interactions', in Bolton, Melvin D.; Thomma, Bart P.H.J. (ed.), Plant Fungal Pathogens: Methods and Protocols, Humana Press, New York, pp. 295-309.
• Zhang, M, Meng, Y, Wang, Q et al 2012, 'PnPMA1, an atypical plasma membrane H+-ATPase, is required for zoospore development in Phytophthora parasitica', Fungal Biology, vol. 116, no. 9, pp. 1013-1023.
• Rafiqi, M, Ellis, J, Ludowici, V et al 2012, 'Challenges and progress towards understanding the role of effectors in plant-fungal interactions', Current Opinion in Plant Biology, vol. 15, pp. 477-482.
• Blackman, L, Arikawa, m, Yamada, S et al 2011, 'Identification of a Mastigoneme Protein from Phytophthora nicotianae', Protist, vol. 162, no. 1, pp. 100-114.
• Koeck, M, Hardham, A & Dodds, P 2011, 'The role of effectors of biotrophic and hemibiotrophic fungi in infection', Cellular Microbiology, vol. 13, no. 12, pp. 1849-1857.
• Whitehead, L, Day, D & Hardham, A 1998, 'Cytoskeletal arrays in the cells of soybean root nodules: The role of actin microfilaments in the organisation of symbiosomes', Protoplasma, vol. 203, no. 3-4, pp. 194-205.
• Gan, P, Rafiqi, M, Ellis, J et al 2010, 'Lipid binding activities of flax rust AvrM and AvrL567 effectors', Plant Signaling & Behavior, vol. 5, no. 10, pp. 1272-1275.
• Gan, P, Rafiqi, M, Hardham, A et al 2010, 'Effectors of biotrophic fungal plant pathogens', Functional Plant Biology, vol. 37, no. 10, pp. 913-918.
• Hardham, A & Blackman, L 2010, 'Molecular cytology of Phytophthora-plant interactions', Australasian Plant Pathology, vol. 39, no. 1, pp. 29-35.
• Rafiqi, M, Gan, P, Ravensdale, M et al 2010, 'Internalization of Flax Rust Avirulence Proteins into Flax and Tobacco Cells Can Occur in the Absence of the Pathogen', The Plant Cell, vol. 22, no. 6, pp. 2017-2032.
• Hardham, A & Cahill, D 2010, 'The role of oomycete effectors in plant-pathogen interactions', Functional Plant Biology, vol. 37, no. 10, pp. 919-925.
• Narayan, R, Blackman, L, Shan, W et al 2010, 'Phytophthora nicotianae transformants lacking dynein light chain 1 produce non-flagellate zoospores', Fungal Genetics and Biology, vol. 47, no. 8, pp. 663-671.
• Dodds, P, Rafiqi, M, Gan, P et al 2009, 'Effectors of biotrophic fungi and oomycetes: pathogenicity factors and triggers of host resistance', New Phytologist, vol. 183, no. 4, pp. 993-1000.
• Gan, P, Shan, W, Blackman, L et al 2009, 'Characterization of cyclophilin-encoding genes in Phytophthora', Molecular Genetics and Genomics, vol. 281, pp. 565-578.
• Hardham, A 2009, 'Functioning Cells', in P Ladiges, B Evans, R Saint, B Knox (ed.), Biology: An Australian Focus (4th ed), McGraw-Hill Inc, Australia, pp. 1-25.
• Harper, J, Thuet, J, Lechtreck, K et al 2009, 'Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates', Protoplasma, vol. 236, pp. 97-101.
• Hardham, A 2009, 'The Asexual Life Cycle', in Kurt Lamour and Sophien Kamoun (ed.), Oomycete Genetics and Genomics: Diversity, Interactions and Research Tools, Wiley-Blackwell, Oxford, pp. 93-119.
• Hardham, A & Shan, W 2009, 'Cellular and Molecular Biology of Phytophthora - Plant Interactions', in Karl Esser, Holger Deising (ed.), The Mycota vol 5: Plant Relationships (2nd ed), Springer, Heidelberg, pp. 3-28.
• Blackman, L & Hardham, A 2008, 'Regulation of catalase activity and gene expression during Phytophthora nicotianae development and infection of tobacco', Molecular Plant Pathology, vol. 9, no. 4, pp. 495-510.
• Hardham, A, Takemoto, D & White, R 2008, 'Rapid and dynamic subcellular reorganization following mechanical stimulation of Arabidopsis epidermal cells mimics responses to fungal and oomycete attack', BMC Plant Biology, vol. 8, no. 63, pp. 1-14.
• Hardham, A 2007, 'Cell Biology of Fungal and Oomycete Infection of Plants', in K Esser (ed.), The Mycota vol VIII, Springer, Germany, pp. 251-290.
• Hardham, A 2007, 'Cell biology of plant-oomycete interactions', Cellular Microbiology, vol. 9, no. 1, pp. 31-39.
• Hardham, A, Jones, D & Takemoto, D 2007, 'Cytoskeleton and cell wall function in penetration resistance', Current Opinion in Plant Biology, vol. 10, pp. 342-348.
• Takemoto, D, Jones, D & Hardham, A 2006, 'Re-organization of the cytoskeleton and endoplasmic reticulum in the Arabidopsis pen1-1 mutant inoculated with the non-adapted powdery mildew pathogen, Blumeria graminis f. sp. hordei', Molecular Plant Pathology, vol. 7, no. 6, pp. 553-563.
• Hardham, A & Takemoto, D 2006, 'Dynamic subcellular responses in plants during interactions with fungi and oomycetes.', in Federico Sanchez, Carmen Quinto, Isabel Lopez-Lara, and Otto Geiger (ed.), Biology of Plant-Microbe Interactions, volume 5, International Society for Molecular Plant-Microbe Interactions, Minnesota, USA, pp. 70-79.
• Shan, W, Hardham, A & Liu, J 2006, 'Phytophthora nicotianae PnPMA1 encodes an atypical plasma membrane H+-ATPase that is functional in yeast and developmentally regulated', Fungal Genetics and Biology, vol. 43, pp. 583-592.
• Skalamera, D & Hardham, A 2006, 'PnCcp, a Phytophthora nicotianae protein containing a single complement control protein module, is sorted into large peripheral vesicles in zoospores', Australasian Plant Pathology, vol. 35, pp. 593-603.
• Jiang, R, Tyler, B, Whisson, S et al 2006, 'Ancient origin of elicitin gene cluster in Phytophthora genomes', Molecular Biology and Evolution, vol. 23, no. 2, pp. 338-351.
• Hardham, A 2005, 'Fungi', Quarterly Review of Biology, vol. 80, no. 1, p. 121.
• Blackman, L, Mitchell, H & Hardham, A 2005, 'Characterisation of manganese superoxide dismutase from Phytophthora nicotianae', Mycological Research, vol. 109, no. 10, pp. 1171-1183.
• Hardham, A 2005, 'Pathogen profile Phytophthora cinnamomi', Molecular Plant Pathology, vol. 6, no. 6, pp. 589-604.
• Takemoto, D, Hardham, A & Jones, D 2005, 'Differences in Cell Death Induction by Phytophthora Elicitins Are Determined by Signal Components Downstream of MAP Kinase Kinase in Different Species of Nicotiana and Cultivars of Brassica rapa and Raphanus sativus', Plant Physiology, vol. 138, pp. 1419-1504.
• Robold, A & Hardham, A 2005, 'During attachment Phytophthora spores secrete proteins containing thrombospondin type 1 repeats', Current Genetics, vol. 47, pp. 307-315.
• Frost, D, Way, H, Howles, P et al 2004, 'Tobacco transgenic for the flax rust resistance gene L expresses allele-specific activation of defense responses', Molecular Plant-Microbe Interactions (MPMI), vol. 17, no. 2, pp. 224-232.
• Hardham, A 2004, 'Fungal and oomycete plant pathogens: Cell biology', in RM Goodman (ed.), Encyclopedia of Plant and Crop Science, Marcel Dekker Inc., New York, pp. 480-483.
• Shan, W, Marshall, J & Hardham, A 2004, 'Gene expression in germinated cysts of Phytophthora nicotianae', Molecular Plant Pathology, vol. 5, no. 4, pp. 317-330.
• Robold, A & Hardham, A 2004, 'Production of monoclonal antibodies against peripheral-vesicle proteins in zoospores of Phytophthora nicotianae', Protoplasma, vol. 223, pp. 121-132.
• Skalamera, D, Wasson, A & Hardham, A 2004, 'Genes expressed in zoospores of Phytophthora nicotianae', Molecular Genetics and Genomics, vol. 270, pp. 549-557.
• Shan, W & Hardham, A 2004, 'Construction of a bacterial artificial chromosome library, determination of genome size, and characterization of an Hsp70 gene family in Phytophthora nicotianae', Fungal Genetics and Biology, vol. 41, pp. 369-380.
• Takemoto, D & Hardham, A 2004, 'The cytoskeleton as a regulator and target of biotic interactions in plants', Plant Physiology, vol. 136, pp. 3864-3876.
• Hardham, A 2004, 'Functioning cells', in P Ladiges, B Evans, R Saint, B Knox (ed.), Biology: An Australian Focus (3rd ed), McGraw-Hill Inc, North Ryde, pp. 67-105.
• Takemoto, D, Jones, D & Hardham, A 2003, 'GFP-tagging of cell components reveals the dynamics of subcellular re-organisation in response to infection of Arabidopsis by oomycete pathogens', The Plant Journal, vol. 33, pp. 775-792.
• Mitchell, H, Kovac, K & Hardham, A 2002, 'Characterization of Phytophthora nicotianae zoospore and cyst membrane proteins', Mycological Research, vol. 106, pp. 1211-1223.
• Ambikapathy, J, Marshall, J, Hocart, C et al. 2002, 'The role of proline in osmoregulation in Phytophthora nicotianae', Fungal Genetics and Biology, vol. 35, pp. 287-299.
• Gotesson, A, Marshall, J, Jones, D et al 2002, 'Characterization and evolutionary analysis of a large polygalacturonase gene family in the oomycete plant pathogen Phytophthora cinnamomi', Molecular Plant-Microbe Interactions (MPMI), vol. 15, no. 9, pp. 907-921.
• Hardham, A 2001, 'Cell biology of fungal infection of plants', in R J Howard and N A R Gow (ed.), The Mycota: A Comprehensive Treatise on Fungi as Experimental Systems for Basic and Applied Research, Volume VIII: Biology of the Fungal Cell, Springer, Berlin, Germany, pp. 91-123.
• Hardham, A 2001, 'Functioning cells', in R B Knox, P Ladiges, B Evans and R Saint (ed.), Biology, McGraw-Hill Inc, Sydney, pp. 70-96.
• Hardham, A 2001, 'Brian E.S. Gunning - on the occasion of his 65th birthday', Protoplasma, vol. 215, pp. 1-3.
• Hardham, A 2001, 'The cell biology of Phytophthera pathogenicity', Australasian Plant Pathology, vol. 30, pp. 91-98.
• Marshall, J, Ashton, A, Govers, F et al 2001, 'Isolation and characterisation of four genes encoding pyruvate, phosphate dikinase in the oomycete plant pathogen Phytophthera cinnamomi', Current Genetics, vol. 40, pp. 73-81.
• Hardham, A 2001, 'Investigations of oomycete cell biology', in N Talbot (ed.), Molecular and Cellular Biology of Filamentous Fungi, Oxford University Press, Oxford, pp. 127-155.
• Marshall, J, Wilkinson, J, Moore, T et al 2001, 'Structure and expression of the genes encoding proteins resident in large peripheral vesicles of Phytopthera cinnamomi zoospores', Protoplasma, vol. 215, pp. 226-239.
• Gautam, Y, Cahill, D & Hardham, A 1999, 'Development of a quantitative immunodipstick assay for Phytophthora nicotianae', Food and Agricultural Immunology, vol. 11, pp. 229-242.
• Mitchell, H & Hardham, A 1999, 'Characterisation of the water expulsion vacuole in Phytophthora nicotianae zoospores', Protoplasma, vol. 206, pp. 118-130.

Projects and Grants

Grants information is drawn from ARIES. To add or update Projects or Grants information please contact your College Research Office.

• Transcriptome analysis of Phytophthora-plant interactions: characterisation of plant inhibitor proteins targeting Phytophthora extracellular effectors (Primary Investigator)
• Molecular basis of rust infection and host plant resistance (Primary Investigator)
• Transcriptome profiling of Phytophthora pathogenicity genes: regulation of cell wall degrading enzyme synthesis during plant infection (Primary Investigator)
• Role of fungal secreted proteins as plant disease effectors (Primary Investigator)