Dr Gaetan Burgio
Areas of expertise
- Genetics 0604
- Quantitative Genetics (Incl. Disease And Trait Mapping Genetics) 060412
- Haematology 110202
- Infectious Diseases 110309
- Genetics Not Elsewhere Classified 060499
- Bacteriology 060501
- Other Biological Sciences 0699
- Medical Molecular Engineering Of Nucleic Acids And Proteins 100403
Research interests
Bacteria or parasite resistant to multiple drugs is a growing concern and affects many patients worldwide every year. There is an urgent need to find novel treatment to improve the care of people as bacteria or parasites are becoming resistant to all existing antimicrobial. Novel and innovative strategies are desperately needed to solve this important problem. Our research aims to better understand how antimicrobial resistance occurs in microorganisms and how to find new therapies.
Our research focuses on three majors themes:
Theme 1: Exploring CRISPR systems diversity
Novel precision genetic technologies such as genome engineering offer novel avenues to a better understanding of the interactions between host and pathogens. Using CRISPR/Cas9, we are able to precisely and efficiently target any modification of the mouse and the pathogen genomes to study genes of interest. We are interested in exploring the diversity of the CRISPR systems and others anti-viral or plasmid defense systems for adaptive immunity. We combine a computational biology with a genetic approach to explore and understand the mechanisms of adaptive immunity in bacteria and harness these systems as gene editing tools.
Theme 2: Uncovering the genetics of the host response to multidrug resistant bacteria
Hospital acquired infections are caused by bacteria, viruses and fungi. These infections are strongly increasing in prevalence to 1 in 25 patients in Europe or in the US. Over 700,000 patients in the US a year contract hospital-acquired infection. Most of these hospital-acquired infections are caused by ‘superbugs’, bacteria that are multi resistant to antibiotics. Superbugs and the lack of novel antibiotics on the market is a growing concern. We are interested in understanding how a multi-resistant bacteria invade, persist in and adapt to the host environment. Our particular interest is the elucidation of the host-pathogens interactions for bacteria such as Enterococcus, Staphylococcus aureus or Acinetobacter baumannii
Theme 3: Investigation of the host response to malaria infection
Malaria infection is the third deadliest disease worldwide and a treatment is desperately needed. Malarial parasites have led to selected mutations showing an increase in the gene pool of human population living in endemic regions and these mutations render the human population more resistant to malaria. We are interested in understanding the mechanisms of resistance to the malaria parasite after invading red blood cells and how the host has developed resistance to the parasite.
Biography
Dr Burgio was born in France and completed his education at Paris. He graduated from the Faculty of Medicine Xavier Bichat in Paris in 2002 with a Medical Doctor Degree. He worked as a consultant in an emergency department at Paris while he was undertaking his PhD studies at Pasteur Institute, Paris. He completed his PhD in 2008 from Pasteur Institute in France and migrated to Australia to join Professor Simon Foote as a postdoctoral researcher at the Menzies Research Institute (University of Tasmania) from 2008 to 2012 and the Australian School of Advanced Medicine at Macquarie University from 2012 to 2015 on the investigation of the host response to the malaria parasite using large scale ENU mitagenesis in mice. In 2015 he was appointed as a group leader at the John Curtin of Medical Research and the head of the transgenesis core facility at the Australian Phenomics Facility to establish a new research program of the host pathogens interaction on multidrug resistant bacteria and the malaria parasite using cutting edge technologies such as CRISPR/Cas9 genome editing. His research is supported from the National Health and Medical Research Council (NHMRC) and the National Collaborative Research Infrastructure Strategy (NCRIS) in Australia.
Publications
- Mathur A, Feng S, Hayward JA, Ngo C, Fox D, Atmosukarto II, Price JD, Schauer K, Märtlbauer E, Robertson AAB, Burgio G, Fox EM, Leppla SH, Kaakoush NO, Man SM. 2019, A multicomponent toxin from Bacillus cereus incites inflammation and shapes host outcome via the NLRP3 inflammasome. Nature Microbiology. https://www.ncbi.nlm.nih.gov/pubmed/30531979
- Burgio, G 2018, 'Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology', https://www.ncbi.nlm.nih.gov/pubmed/29490686
- Cardinez, C, Miraghazadeh, B, Tanita, K et al 2018, 'Gain-of-function IKBKB mutation causes human combined immune deficiency', https://www.ncbi.nlm.nih.gov/pubmed/30337470
- Conway, A, Brown, F, Hortle, E et al 2018, 'Bone marrow transplantation corrects haemolytic anaemia in a novel ENU mutagenesis mouse model of TPI deficiency', https://www.ncbi.nlm.nih.gov/pubmed/29720471
- Faunce, T, Ray, A, Gardiner, C et al 2018, 'Regulating RNA Research and CRISPR Gene Drives to Combat Biosecurity Threats', https://www.ncbi.nlm.nih.gov/pubmed/30302983
- Huang, H, McMorran, B, Foote, S et al 2018, 'Host genetics in malaria: lessons from mouse studies', https://www.ncbi.nlm.nih.gov/pubmed/29594458.
- Lelliott, P, Huang, H, Dixon, M et al 2018, 'Erythrocyte β spectrin can be genetically targeted to protect mice from malaria', https://www.ncbi.nlm.nih.gov/pubmed/29296915.
- Huang, H, Bauer, D, Lelliott, P et al 2017, 'Ankyrin-1 gene exhibits allelic heterogeneity in conferring protection against Malaria', https://www.ncbi.nlm.nih.gov/pubmed/28751503.
- Khin, N, Lowe, J, Starrs, L et al 2017, 'No evidence for genome editing in mouse zygotes and HEK293T human cell line using the DNA-guided Natronobacterium gregoryi Argonaute (NgAgo)', https://www.ncbi.nlm.nih.gov/pubmed/28609472
- Menon, D, Innes, A, Oakley, A et al 2017, 'GSTO1-1 plays a pro-inflammatory role in models of inflammation, colitis and obesity', https://www.ncbi.nlm.nih.gov/pubmed/29259211
- Hortle, E, Nijagal, B, Bauer, D et al 2016, 'Adenosine monophosphate deaminase 3 activation shortens erythrocyte half-life and provides malaria resistance in mice', https://www.ncbi.nlm.nih.gov/pubmed/27465915.
- Huang, H, Bauer, D, Lelliott, P et al 2016, 'A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice', https://www.ncbi.nlm.nih.gov/pubmed/27848995.
- Laissue, P, Lakhal, B, Vatin, M et al 2016, 'Association of FOXD1 variants with adverse pregnancy outcomes in mice and humans', https://www.ncbi.nlm.nih.gov/pubmed/27805902.
- Bauer, D, McMorran, B, Foote, S et al 2015, 'Genome-wide analysis of chemically induced mutations in mouse in phenotype-driven screens', http://www.ncbi.nlm.nih.gov/pubmed/26503232
- Dogovski, C, Xie, S, Burgio, G et al 2015, 'Targeting the Cell Stress Response of Plasmodium falciparum to Overcome Artemisinin Resistance' http://www.ncbi.nlm.nih.gov/pubmed/25901609
- Lelliott, P, McMorran, B, Foote, S et al 2015, 'Erythrocytic iron deficiency enhances susceptibility to Plasmodium chabaudi infection in mice carrying a missense mutation in transferrin receptor 1', http://www.ncbi.nlm.nih.gov/pubmed/26303393
- Lelliott, P, McMorran, B, Foote, S et al 2015, 'In vivo assessment of rodent Plasmodium parasitemia and merozoite invasion by flow cytometry', http://www.ncbi.nlm.nih.gov/pubmed/25867202
- Lelliott, P, McMorran, B, Foote, S et al 2015, 'The influence of host genetics on erythrocytes and malaria infection: Is there therapeutic potential?', http://www.ncbi.nlm.nih.gov/pubmed/26215182
- Phillips, J, Boyd, R, Krockenberger, M et al 2015, 'Progression of anemia and its relationship with renal function, blood pressure, and erythropoietin in rats with chronic kidney disease', http://www.ncbi.nlm.nih.gov/pubmed/26292198
- Brizuela, M, Huang, H, Smith, C et al 2014, 'Treatment of erythrocytes with the 2-Cys peroxiredoxin inhibitor, conoidin A, prevents the growth of Plasmodium falciparum and enhances parasite sensitivity to chloroquine', http://www.ncbi.nlm.nih.gov/pubmed/24699133
- Lelliott, P, Lampkin, S, McMorran, B et al 2014, 'A flow cytometric assay to quantify invasion of red blood cells by rodent Plasmodium parasites in vivo', http://www.ncbi.nlm.nih.gov/pubmed/24628989
- McMorran, B, Burgio, G & Foote, S 2013, 'New insights into the protective power of platelets in malaria infection' http://www.ncbi.nlm.nih.gov/pubmed/23710276
- Burgio, G, Baylac, M, Heyer, E et al 2012, 'Exploration of the genetic organization of morphological modularity on the mouse mandible using a set of interspecific recombinant congenic strains between C57BL/6 and mice of the mus spretus species', http://www.ncbi.nlm.nih.gov/pubmed/23050236
- Burgio, G, Baylac, M, Heyer, E et al 2012, 'Nasal bone shape is under complex epistatic genetic control in mouse interspecific recombinant congenic strains', http://www.ncbi.nlm.nih.gov/pubmed/22662199
- Greth, A, Lampkin, S, Mayura-Guru, P et al 2012, 'A novel ENU-mutation in ankyrin-1 disrupts malaria parasite maturation in red blood cells and mice' http://www.ncbi.nlm.nih.gov/pubmed/22723917
- McMorran, B, Wieczorski, L, Drysdale, K et al 2012, 'Platelet factor 4 and duffy antigen required for platelet killing of Plasmodium falciparum' http://www.ncbi.nlm.nih.gov/pubmed/23224555
- Vatin, M, Burgio, G, Renault, G et al 2012, 'Refined mapping of a quantitative trait locus on chromosome 1 responsible for mouse embryonic death', http://www.ncbi.nlm.nih.gov/pubmed/22916247
- Longley, R, Smith, C, Fortin, A et al 2010, 'Host resistance to malaria: Using mouse models to explore the host response' http://www.ncbi.nlm.nih.gov/pubmed/21116636
Projects and Grants
Grants information is drawn from ARIES. To add or update Projects or Grants information please contact your College Research Office.
- Myelin Plasticity - How Does it Work? (Secondary Investigator)
- Elucidating the tumour suppressor behaviour of FUBP1 in glioma (Secondary Investigator)
- ENU Mutagenesis and malaria chemotherapy (Secondary Investigator)
- How platelets kill malaria parasites (Secondary Investigator)